FastDNA®SPIN Kit for Soil 全球最快速、最高效的土壤DNA提取、纯化试剂盒 优势： 配合FastPrep®样品处理系统，40秒内裂解样品，30分钟内完成DNA提取 通过简单的仪器裂解、离心、过滤、洗脱等过程，即可获得纯化后的DNA样品 超高的DNA得率，特别适合于分子生态学研究 适用于不同类型的土壤中微生物基因组DNA的分离 不含任何有毒有害的有机试剂成分 产品简介 FastDNA®Spin Kit土壤DNA试剂盒适用于从土壤和其它环境样品中的细菌、真菌、动植物提取基因组DNA.每个裂解管可处理500mg土壤样品，运用基于硅吸附DNA原理的GENECLEAN®法对粗提的DNA样品进行纯化，得到的DNA可直接用于PCR和其它实验。 试剂盒组成 裂解研磨粉E，纯化吸附硅砂，SEWS-M溶液，DES溶液，BBS溶液，磷酸钠缓冲液，PPS溶液，MT溶液，收集管，吸附柱 The FastDNA® SPIN Kit for Soil quickly and efficiently isolates PCR-ready genomic DNA directly from soil samples in less than 30 minutes. Designed for use with the FastPrep® Instruments from MP Biomedicals, plant and animal tissues, bacteria, algae, fungi spores and other members of a soil population are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by multidirectional, simultaneous impaction with lysing matrix particles. FastPrep® Instruments provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods using enzymatic digestion, sonication, blending, douncing and vortexing. Samples are placed into 2.0 ml tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi. Homogenization in the FastPrep® Instrument with Lysing Matrix E takes place in the presence of MT Buffer and Sodium Phosphate Buffer, reagents carefully developed to protect and solubilize nucleic acids and proteins upon cell lysis. These reagents work together to allow extraction of genomic DNA with minimal RNA contamination. Following lysis, samples are centrifuged to pellet soil, cell debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for PCR, restriction digest, electrophoresis and any other desired application.