Cystatin C Human ELISA Kit

• Use - Kidney Injury Marker, Results in 2 Hours
• Sample - Serum, Plasma, Urine or TCM
• Samples/Kit - 40 in Duplicate
• Stability - Stable 4˚C Liquid Reagents

• 循环中的半胱氨酸蛋白酶抑制剂C(Cystatin C，Cys-C)仅经肾小球滤过而被清除，是一种反映肾小球滤过率变化的内源性标志物。因其分子量小，能自由通过肾小球滤过膜，几乎完全被肾小管重吸收且不被分泌，能很好的替代肌酐而成为一种新的反映GFR的理想标志物。

  Cys-C是目前发现的对组织蛋白酶B抑制作用最强的物质，其生物学功能主要是调节半胱氨酸蛋白酶活性。随着科学家对Cys-C的深入研究，发现其不仅在肾脏疾病方面有临床价值，而且还揭示了它与肝脏疾病、糖尿病、肿瘤等具有一定的相关性.

  ARBOR ASSAY公司推出的半胱氨酸蛋白酶抑制剂检测试剂盒（CystatinC Human ELISA Kit）将会很好的辅助您肾脏方面的研究。
  

  Cystatin C Human ELISA Kit具有以下几个特点：

• 用途------检测肾损伤特异性标志物;

• 应用范围广------能够用于检测人来源的血清、血浆、尿液以及组织培养基中的样本;

• 实验操作简单------能够实现2小时内完成样本的检测。

The DetectX^® Cystatin C Immunoassay Kit is designed to measure Human Cystatin C levels in a variety of biological matrices. This kit uses a microtiter plate coated with a monoclonal to Cystatin C and a native human Cystatin C molecule standard. Samples and standards are added to the plate and after an hour incubation at RT the plate is washed and monoclonal antibody-peroxidase conjugate is added. After a 30-minute incubation at RT, the plate is washed and TMB substrate solution added to each well. The color reaction is read at 450nm.

Cystatin C is a non-glycosylated protein of low molecular weight (13kDa) in the cystatin superfamily. Cystatin C is produced at a constant rate in all nucleated cells. Cystatin C belongs to the cysteine proteinase inhibitor group and is associated with several pathological conditions. Imbalance between Cystatin C and cysteine proteinases is associated with diseases such as inflammation, renal failure, cancer, Alzheimer’s disease, multiple sclerosis and hereditary Cystatin C amyloid angiopathy. Cystatin C is removed from blood plasma by glomerular filtration in the kidneys. It is reabsorbed by the proximal tubular cells and degraded. There is a linear relationship between the reciprocal Cystatin C concentration in plasma and the glomerular filtration rate (GFR). Cystatin C is suggested to be a better marker for GFR than other markers as its serum concentration is not affected by other factors such as age, gender and body mass. There is association of Cystatin C levels with the incidence of myocardial infarction and coronary death, presenting a risk factor for secondary cardiovascular events.