DNA甲基化转移酶3B活性/抑制分析试剂盒

¥1
美国eBioscince
美国/中国
2021-07-15 17:44

上海信裕生物科技有限公司

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上海信裕生物科技有限公司
徐云云
15221858802 021-60526354
shxysw02@163.com
产品属性
保存条件4℃保存
保质期6个月
英文名DNA Methyltransferase 3B Activity/Inhibitor
数量1
供应商上海信裕
产品说明
  • DNA甲基化转移酶3B活性/抑制分析试剂盒详细信息:
  • Product Overview

    The EpiQuik™ DNA Methyltransferase Activity/Inhibitor Screening Assay Kit is a convenient set of tools that allows the experimenter to selectively screen Dnmt3B activity/inhibitors. The selective inhibition of Dnmt3B may lead to demethylation and expression of the silenced tumor suppressor genes. Thus the selective Dnmt3B inhibitors could be new addition to cancer therapeutic agents. The kit is ready-to-use and provides all the essential components needed to carry out a successful Dnmt3B activity/inhibitor screening experiment without the need for radioactivity or any special equipment. 

    WHY CHOOSE THE EPIQUIK™ DNA METHYLTRANSFERASE 3B ACTIVITY/INHIBITOR SCREENING ASSAY KIT?
    • Very rapid procedure, which can be completed within 3 hours.
    • Safe and innovative colorimetric assay without radioactivity, extraction, and chromatography.
    • Strip microplate format makes the assay flexible: manual or high throughput analysis.
    • Extremely simple, reliable, and consistent assay conditions.
    DNA甲基化转移酶3B活性/抑制分析试剂盒Principle & Procedure


    The EpiQuik™ DNA Methyltransferase 3B Activity/Inhibitor Screening Assay Kit is designed for screening Dnmt 3B inhibitors. In an assay with this kit, the unique cytosine-rich DNA substrate is stably coated on the strip wells. These wells are specifically treated to have a high DNA absorption ability. Dnmt 3B enzymes transfer methyl group to cytosine from Adomet to methylate DNA substrate. The methylated DNA can be recognized with anti-5-methylcytosine antibody. The ratio or amount of methylated DNA, which is proportional to enzyme activity, can then be colorimetrically quantified through an ELISA-like reaction.

    SCHEMATIC PROCEDURE:
    DNA甲基化转移酶3B活性/抑制分析试剂盒Product Components

    MT1 (10x wash buffer)
    MT2 (Dnmt assay buffer)
    MT3 (Adomet)*
    MT4 (active Dnmt3B, 200 µg/ml)
    MT5 (capture antibody )*
    MT6 (detection antibody )*
    MT7 (developing solution)
    MT8 (stop solution)
    8 well substrate-coated strip (with frame)
    User guide
    xy505-2 HRP标记生物素
    xy506-1 生物素化羊抗人IgA
    xy506-2 生物素化羊抗人IgA
    xy507-1 生物素化羊抗人IgG
    xy507-2 生物素化羊抗人IgG
    xy507-3 生物素化羊抗人IgG
    xy508-1 生物素化羊抗人C3
    xy508-2 生物素化羊抗人C3
    xy509-1 生物素化羊抗兔IgG
    xy509-2 生物素化羊抗兔IgG
    xy509-3 生物素化羊抗兔IgG
    xy510-1 生物素化兔抗羊IgG
    xy510-2 生物素化兔抗羊IgG
    xy511-1 生物素化羊抗鼠IgG
    xy511-2 生物素化羊抗鼠IgG
    xy511-3 生物素化羊抗鼠IgG
    xy512-1 生物素化兔抗鸡IgG(Y)
    xy512-2 生物素化兔抗鸡IgG(Y)
    xy513-1 生物素化羊抗大鼠IgG
    xy513-2 生物素化羊抗大鼠IgG
    xy601-1 羊抗人IgG
    xy601-2 羊抗人IgG
    xy602-1 兔抗羊IgG
    xy602-1 兔抗羊IgG
    xy603-1 羊抗人IgA(α链特异)
    xy603-2 羊抗人IgA(α链特异)
    xy604-1 兔抗豚鼠IgG
    xy604-2 兔抗豚鼠IgG
    xy605-1 羊抗人IgM(μ链特异)
    xy605-2 羊抗人IgM(μ链特异)
    xy606-1 兔抗大鼠IgG
    xy606-2 兔抗大鼠IgG
    xy607-1 羊抗兔IgG
    xy607-2 羊抗兔IgG
    * For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap.
    DNA甲基化转移酶3B活性/抑制分析试剂盒User Guide & MSDS