性质

产品描述

Application

甲酰胺可使核酸双螺旋不稳定，通常可以 50% 的浓度，用于需要较低杂交温度的杂交实验。

Formamide is suitable for use in hybridization buffers made using standard recipes. It was used in preparation of brain sections of mice for BrdU immunohistochemistry^[2]and FISH analysis of HeLa cells.^[3] It was used to prepare the polyacrylamide gel for analysis of PCR products using denaturing gradient gel electrophoresis.^[1]

General description

Formamide is a clear liquid amide derived from formic acid. It can be used as a cryoprotectant and gel-stabilizer. It is known to destabilize nucleic acid duplexes, making it useful for preparing hybridization buffers for RNA and DNA applications.

Other Notes

The product has been deionized for molecular biology use and packaged under argon gas in amber glass bottles to protect it from oxidation.