Fig1: ICC staining Fascin(ET1705-18)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed cytoplasm staining on Hela cells.

Fig2: ICC staining SMC3(ET1703-35)(1/100) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ARITC-conjugatedoat anti-rabbit IgG&L (HA1016) (1/100)as used as the secondary antibody. The result showed nuclear staining on Hela cells.

Fig3: IF analysis of 4% formalin-fixed, 0.2% Triton X-100 permeabilized human spleen tissue labeling CD34 with (ET1606-11) at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (TRITC) (HA1016) secondary antibody at 1/200 dilution (red). The nuclear counterstain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/200 dilution.

Fig4: Flow cytometric analysis of Hela cells with SMC3 (ET1703-35) antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is HA1016 at 1/100 dilution.