Introduction
Interleukin-1β (IL-1β) has a wide spectrum of inflammatory, metabolic, haemopoietic, and immunological properties (1). IL-1β plays a significant role in hippocampal synaptic function (2) and is a potential genetic marker as indicator of gastric cancer risk (3). High plasma level of interleukin-1β is associated with rheumatoid and osteoarthritic joint disease (4), infectious gastroenteritis (5), neurodegeneration (6), and breast cancer (7). High gingival crevicular fluid levels of IL-1β are related to type 2 diabetes (8).

Principle of the Assay
The AssayMax Mouse Interleukin-1β ELISA kit is designed for detection of mouse IL-1β in plasma, serum, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures IL-1β in less than 5 hours. A murine monoclonal antibody specific for IL-1β has been pre-coated onto a microplate. IL-1β in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for IL-1β, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.