本分离液为Ficoll、羟乙基淀粉550与泛影酸葡jia胺的混合液。抗凝外周血可在分离液中分层。离心时,在Ficoll、羟乙基淀粉的作用下红细胞与粒细胞聚集并迅速沉降;此时,淋巴细胞及其他单个核细胞仍处于分离液上层,红细胞污染可忽略不计。大部分血小板可在细胞清洗低速离心过程中去除。 其他人及动物多种比重细胞分离液,因不同种属不同比重分离液的细胞离散系数及细胞带电不同,用户在制定分离液时应提供所需分离液的比重、动物种属及被分离细胞的名称。
适用于从动物抗凝血液中分离淋巴细胞,无菌条件下所分离的淋巴细胞可用于细胞培养等。本品仅供科研使用。
本品易感染细菌,需无菌条件操作。无菌条件下操作,启封后置常温保存。如 4℃保存,本分离液易出现白色结晶,影响分离效果。

参考文献:
《The antiviral property of Sargassum fusiforme polysaccharide for avian leukosis virus subgroup J in vitro and in vivo》 作者:YuhaoSunabcXiaolinChenabLiliZhangdHongLiuabcSongLiuabHuahuaYuabXueqinWangabYukunQinabPengchengLi 期刊:International Journal of Biological Macromolecules 影响因子:4.784 PMID:31306705
《A polysaccharide found in Paulownia fortunei flowers can enhance cellular and humoral immunity in chickens》 作者:Qiuju Wang,Xiuyan Meng,Lin Zhu, Yulin Xu,Wenping Cui,Xiaohua He,Kai Wei,Ruiliang Zhu 期刊:Int. J. Biol. Macromol. 影响因子:4.784 PMID:30708021
《Combined adjuvant effect of ginseng stem-leaf saponins and selenium on immune responses to a live bivalent vaccine of Newcastle disease virus and infectious bronchitis virus in chickens》 作者:X Ma, S Bi, Y Wang, X Chi, S Hu 期刊:Poult. Sci. 影响因子:2.027 PMID:31220864
《Construction of a novel DNA vaccine candidate targeting F gene of genotype VII Newcastle disease virus and chicken IL‐18 delivered by Salmonella》 作者:X. Gao,K. Xu,G. Yang,C. Shi,H. Huang,J. Wang,W. Yang,J. Liu,Q. Liu,Y. Kang,Y. Jiang,C. Wang 期刊:J. Appl. Microbiol. 影响因子:2.683 PMID:30785663
《Effects of Diutina rugosa SD-17 on growth performance, intestine morphology, and immune status of chickens》 作者:J Wang, B Wang, H Du, H Zhang, H Li, F Wang, X Zhao 期刊:Poult. Sci. 影响因子:2.027 PMID:31350991
《Effect of in ovo feeding of vitamin C on antioxidation and immune function of broiler chickens》 作者:Y. F. Zhu , S. Z. Li, Q. Z. Sun and X. J. Yang 期刊:Animal 影响因子:2.026 PMID:30621801
《Altered gene expression profiles of the MDA5 signaling pathway in peripheral blood lymphocytes of chickens infected with avian reovirus》 作者:Liji Xie,Zhixun Xie,Sheng Wang,Jiaoling Huang,Xianwen Deng,Zhiqin Xie,Sisi Luo,Tingting Zeng,Yanfang Zhang,Minxiu Zhang 期刊:Arch. Virol. 影响因子:2.261 PMID:31273469
《Cholesterol-25-hydroxylase Is a Chicken ISG That Restricts ALV-J Infection by Producing 25-hydroxycholesterol》 作者:Tingting Xie , Min Feng, Manman Dai , Guodong Mo , Zhuohao Ruan , Guiyan Wang , Meiqing Shi and Xiquan Zhang 1,2,*OrcID 期刊:Viruses 影响因子:3.811 PMID:31151272
《A balanced game: chicken macrophage response to ALV-J infection》 作者:Min Feng, Tingting Xie, Yuanfang Li, Nan Zhang, Qiuyuan Lu, Yaohong Zhou, Meiqing Shi, Jingchen Sun & Xiquan Zhang 期刊:Vet. Res. 影响因子:3.117 PMID:30841905