| 数量 | 现货 |
| 供应商 | 博士德生物 |
| 检测方法 | 酶联免疫双抗夹心法 |
| 应用 | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 适应物种 | Human |
| 规格 | 96T |
| 货号 | EK0439 |
|---|---|
| 产品名称 | Human Leptin receptor PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human Leptin receptor in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, T20-D839 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <20pg/ml |
| 检测范围 | 312pg/ml-20000pg/ml |
| 基因名称 | LEPR |
| 基因全名 | leptin receptor |
| Uniprot ID | P48357 |
| 指标别名 | CD295|HuB219|LEP-R|LEPR|LEPR_HUMAN|Leptin receptor|OB receptor|OB-R|OBR |
| 背景资料 | Leptin receptor(or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus. Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family. Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene(LEPR) might contribute to obesity or obesity-related diseases. Leptin is also involved in the regulation of blood pressure through the leptin receptor. |
| 显色时间 | 25-30 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0439-CAP | 96-well plate precoated with anti- human Leptin receptor antibody | 1 |
| EK0439-ST | Lyophilized recombinant human Leptin receptor standard | 20ng/tubex2 |
| EK0439-DA | Biotinylated anti- human Leptin receptor antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 508 | 1680 | 9187 | 484 | 1787 | 9762 |
| Standard deviation | 30.98 | 90.72 | 404.22 | 37.75 | 128.66 | 429.52 |
| CV(%) | 6.1% | 5.4% | 4.4% | 7.8% | 7.2% | 4.4% |
| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.047 | 0.116 | 0.169 | 0.265 | 0.506 | 0.927 | 1.534 | 2.375 |
