The Invitrogen™ Anza™ T4 PNK (polynucleotide kinase) Kit is used to perform 5’-phosphorylation of DNA and oligonucleotides. T4 PNK exhibits 5’ polynucleotide kinase and 3’ phosphatase activity, catalyzing the transfer of the terminal phosphate of ATP to a 5’ hydroxyl group of a nucleic acid.
When preparing DNA for the ligation step in cloning, either the insert DNA or the vector DNA should contain a 5’ phosphate. Often PCR-amplified DNA is treated with T4 PNK to phosphorylate the 5’ end for subsequent cloning ligation.
Benefits: • Fast 15-minute incubation at room temperature • Phosphorylaton can be performed with DNA or oligonucleotides in water, TE, or 1X Anza buffers
The Anza T4 PNK Kit is part of the Anza™ Restriction Enzyme Cloning System, unifying the traditional cloning processes. Other Anza DNA Modifying Enzymes include the Anza T4 DNA Ligase Master Mix, Anza DNA Blunt End Kit, and Anza DNA End Repair Kit.
