Cholesterol Esterase, chemically modified 胆固醇酯酶-11641735103
from Pseudomonas species, lyophilizate
Hydrolase that splits fatty acids from sterols.
Application
Use Cholesterol Esterase, chemically modified in diagnostic tests for the deter-
mination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos.
11 479 709 103, 10 634 522 103 or 10 129 054.
Benefits
Take advantage of the enhanced stability of this enzyme in liquid re- ■
agents.
Rely on the proven diagnostic quality of this product. ■
EC 3.1.1.13
Properties
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10 -5 mol/l
Inhibitors: Heavy metals such as Cu 2+ , Ag + , Zn 2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure)
Temperature dependence: Not possible to determine under assay conditions
due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure)
Thermal stability: Below +20°C (see figure)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives
complete
cleavage of all serum cholesterol esters.
Specification
Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/ml)
pH value (c=50 mg/ml, in water): 7.0-8.0
Activity (+25°C, cholesterol oleate): ≥10 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Volume Activity: For information only
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤1.00
Glycerokinase: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.001
Uricase: ≤0.005
NaCl: 3±0.2 mol/l
Stability: At +2 to +8°C within specification range for 12 months. Store dry.