【别名】

DDBB; FLJ34321; UV-DDB2;

【产品描述】

Damaged DNA-Binding Protein (DDB) consists of a 127 kDa subunit (DDB-1) and a 48 kDa subunit (DDB-2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB) (1-3). In conjunction with CUL4A and ROC-1, the UV-DDB complex forms an E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway (4-6). Loss of DDB activity has been identified in a subset of xeroderma pigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients (7-10).

【基因号】

1643

【亚型】

IgG

【预测分子量】

48kDa

【目标蛋白】

Recombinant protein of human DDB2

【成分】

PBS with 0.02% sodium azide, 50% glycerol, pH7.534.

【贮存方法】

Store at -20°C (regular) or -80°C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

【推荐稀释度】

Western Blot 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Western blot analysis of extracts of MCF-7 cell line, using DDB2 antibody.

Immunohistochemistry of paraffin-embedded rat heart using DDB2 antibody at dilution of 1:100 (x400 lens).

Immunofluorescence analysis of MCF7 cell using DDB2 antibody. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cell using DDB2 antibody. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.

【说明书】Golden Grade Anti-DDB2说明书