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| 数量 | 现货 |
| 供应商 | 博士德生物 |
| 检测限 | 7.8pg/ml-500pg/ml |
| 检测方法 | 酶联免疫双抗夹心法 |
| 应用 | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 适应物种 | Mouse |
| 规格 | 96T |
| 货号 | EK0566 |
|---|---|
| 产品名称 | Mouse CCL1/TCA3 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of mouse CCL1 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, K24-C92 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <1pg/ml |
| 检测范围 | 7.8pg/ml-500pg/ml |
| 基因名称 | CCL1 |
| 基因全名 | chemokine (C-C motif) ligand 1 |
| Uniprot ID | P10146 |
| 指标别名 | C-C motif chemokine 1|CCL1|chemokine (C-C motif) ligand 1|I-309|inflammatory cytokine I-309|P500|SCYA1|SISe|small inducible cytokine A1 (I-309, homologous to mouse Tca-3)|small-inducible cytokine A1|T lymphocyte-secreted protein I-309|TCA3 |
| 背景资料 | CCL1, Chemokine(C-C motif) ligand 1, is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine receptor CCR8. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0566-CAP | 96-well plate precoated with anti- mouse CCL1 antibody | 1 |
| EK0566-ST | Lyophilized recombinant mouse CCL1 standard | 10ng/tubex2 |
| EK0566-DA | Biotinylated anti- mouse CCL1 antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 17 | 81 | 291 | 16 | 78 | 277 |
| Standard deviation | 0.9 | 5.5 | 18.91 | 1.02 | 6.78 | 20.22 |
| CV(%) | 5.3% | 6.8% | 6.5% | 6.4% | 8.7% | 7.3% |
| Concentration(pg/ml) | 0 | 7.8 | 15.6 | 31.3 | 62.5 | 125 | 250 | 500 |
| O.D. | 0.029 | 0.098 | 0.157 | 0.318 | 0.574 | 1.144 | 1.630 | 2.067 |
