Leica DM6000 B with Leica Structured Illumination
Comparison between widefield (left) and Structured Illumination (right)
Montage of 6x4 tiles with 10x/0.4
Scan area: 4.8 x 5.3mm per embryo
Using Leica MM AF Auto Scan-slide
beta-Tubulin: Alexa633
Sample: Courtesy of Dr. Yves Lutz, Imaging Center IGBMC, France
Leica DM6000 B with Leica Structured Illumination
Stitched mosaic of 3x2 images
Stains: DCX-Cy3
Objective: 100x/1.4 Oil
63x Oil Immersion Objective
Red: Phalloidin
Green: wheat germ agglutinin
Maximum projection of a stack containing 65 planes.
Green: glomeruli and convoluted tubules (wheat germ agglutinin Alexa Fluor® 488)
Blue: Nuclei (DAPI).