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常用规格

• Facilitated expansion of human embryonic stem cells by single-cell enzymatic dissociation.

作者

Ellerström C, Strehl R, Noaksson K, Hyllner J, Semb H

杂志

Stem Cells (2007) 25:1690-1696

产品用途

TrypLE select is better than trypsin for passage of human ES cells, which increases clone survival.

ID:

PN61410

• Electrophoretic profiling of both RNA and protein from a single 250-pL sample.

作者

Zabzdyr Jennifer L; Lillard Sheri J;

杂志

Anal Chem (2002) 74:1857-1862

产品用途

Gibco (Invitrogen) 0.25% Trypsin-EDTA was used to remove Cho-K1 cells from cell culture flasks. Gibco (Invitrogen) penicillin/streptomycin was present in the Cho-K1 cell growth medium to prevent bacterial growth. Gibco F-12K (Kaighn's modification) is optimal for growing Cho-K1 cells.

ID:

PN65768

• Characterization of the 46-kDa intermediates of matrix metalloproteinase 3 (stromelysin 1) obtained by site-directed mutation of phenylalanine 83.

作者

Benbow U; Butticè G; Nagase H; Kurkinen M;

杂志

J Biol Chem (1996) 271:10715-10737

产品用途

ProMMP-3 (the precursor of matrix metalloproteinase 3) and its mutant cDNA constructs were stably transfected into CHOK-1 cells using Lipofectin. Transfected cells were initially grown in glutamine-free Dulbecco's modified Eagles's medium containing dialyzed FBS and 25 µM methionine sulfoximine for 2 weeks. After colony formation, cells were split into 24-well plates and grown for additional 2 weeks in same medium with increasing concentrations of methionine sulfoximine (100-400 µM).

ID:

PN60108