本公司提供科研Cyclin A2/CCN1周期素A2抗体，抗体质量可靠，订购Cyclin A2/CCN1周期素A2抗体请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：周期素A2抗体
英文名称：Anti-Cyclin A2/CCN1
货号：bs-0571R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 47kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：Cyclin is essential for the control of the cell cycle at the G1/S (start) and the G2/M (mitosis) transitions. Interacts with the CDK2 and CDC2 protein kinases to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex. When associated with CDK2 (but not with CDC2), interacts with SCAPER. Subcellular location in Nucleus. Cytoplasm. Belongs to the cyclin family. Cyclin AB subfamily.Cyclin A 可以出现在所有类型的细胞。各周期素可出现在不同的细胞增殖周期中。能结合和激活CDK2激酶，促进细胞G1/S和G2/M增殖周期的转化。产品图片Tissue/cell: mouse lymphoma tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin A2 Polyclonal Antibody, Unconjugated(bs-0571R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingTissue/cell: human bile duct cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cyclin A2 Polyclonal Antibody, Unconjugated(bs-0571R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingAntigen: bs-0571P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; TMB(C-0024) staining; Read the data in MicroplateReader by 450nm.