Mouse CCL9 PicoKine ELISA Kit价格,详情介绍-960化工网

Mouse CCL9 PicoKine ELISA Kit

价格：￥2280

品牌：BOSTER

产地：武汉

最新更新日期：2021-07-26 15:18

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联系电话： 027-67845390

联系邮箱：boster@boster.com.cn

产品属性

数量

现货

供应商

博士德生物

检测限	7.8pg/ml-500pg/ml

检测方法

酶联免疫双抗夹心法

应用	cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).

适应物种

Mouse

规格

96T

产品说明

货号	EK1225
产品名称	Mouse CCL9 PicoKine ELISA Kit
产品描述	For quantitative detection of mouse CCL9 in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
免疫原	E.coli, Q22-Q122
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<1pg/ml
检测范围	7.8pg/ml-500pg/ml
基因名称	Ccl9
基因全名	chemokine (C-C motif) ligand 9
Uniprot ID	P51670
指标别名	CCF18\|CCL9\|Chemokine CC motif ligand 9\|Macrophage Inflammatory Protein 1 gamma\|Macrophage inflammatory protein-related protein 2\|MIP-1 gamma\|mMIP1g\|SCYA10\|SCYA9\|Small inducible cytokine A9
背景资料	Chemokine(C-C motif) ligand 9(CCL9), also called MIP-1 gamma and MRP-2, is a small cytokine belonging to the CC chemokine family. CCL9 which found around Peyer's patches is secreted by follicle-associated epithelium(FAE), and it can attract dendritic cells that possess the cell surface molecule CD11b and the chemokine receptor CCR1. CCL9 is constitutively expressed in macrophages and myeloid cells. CCL9 can activate osteoclasts through its receptor CCR1(the most abundant chemokine receptor found on osteoclasts) suggesting an important role for CCL9 in bone resorption.
显色时间	15-20

SKU	名称	数量
Catalog number	Description	Quantity
EK1225-CAP	96-well plate precoated with anti- mouse CCL9 antibody	1
EK1225-ST	Lyophilized recombinant mouse CCL9 standard	10ng/tube×2
EK1225-DA	Biotinylated anti- mouse CCL9 antibody	130μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	130μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

(TMB 孵育条件： 37°C， 15-20)

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.030	0.111	0.197	0.369	0.680	1.252	1.783	2.150

*典型数据仅供参考。用户需要自己建立标准曲线。