适用生物 Homo sapiens (人，小鼠，大鼠)
细胞外信号调节激酶1(ERK1)多克隆抗体产品应用 WB, ICC, IHC-P, IHC-F, ELISA 规格 100ug
标记物 未标记 价格 请咨询当地代理商
浓度 200ug/ml
克隆类型 多克隆 宿主 Rabbit，兔
细胞外信号调节激酶1(ERK1)多克隆抗体Polyclonal Antibody to Extracellular Signal Regulated Kinase 1 (ERK1)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | PAB357Hu01 |
| Clonality | Polyclonal |
| Host | Rabbit |
| Immunoglobulin Type | IgG |
| Purification | Affinity Chromatography |
| Applications | WB, ICC, IHC-P, IHC-F, ELISA |
| Concentration | 200ug/ml |
| UOM | 100ug |
| Conjugate | No Conjugate |
IMMUNOGEN INFORMATION
细胞外信号调节激酶1(ERK1)多克隆抗体Immunogen: Recombinant ERK1 (Pro75~Thr312) expressed in E.coli.
Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- PFEHQT YCQRTLREIQ ILLRFRHENV IGIRDILRAS TLEAMRDVYI VQDLMETDLY KLLKSQQLSN DHICYFLYQI LRGLKYIHSA NVLHRDLKPS NLLINTTCDL KICDFGLARI ADPEHDHTGF LTEYVATRWY RAPEIMLNSK GYTKSIDIWS VGCILAEMLS NRPIFPGKHY LDQLNHILGI LGSPSQEDLN CIINMKARNY LQSLPSKTKV AWAKLFPKSD SKALDLLDRM LT
ANTIBODY SPECIFITY
The antibody is a rabbit polyclonal antibody raised against ERK1. It has been selected for its ability to recognize ERK1 in immunohistochemical staining and western blotting.
APPLICATIONS
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
CONTENTS
Form & Buffer: Supplied as solution form in PBS, pH7.4,
containing 0.02% NaN3, 50% glycerol.
IHC Images
Used in DAB staining on fromalin fixed paraffin-embedded Liver tissue
QUALITY CONTROL
细胞外信号调节激酶1(ERK1)多克隆抗体Content: The quality control contains recombinant ERK1 (Pro75~Thr312) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
STORAGE
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
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