ZYMO DNA/RNA Shield,DNA/RNA保存液

¥3400
ZYMO
2021-07-30 13:27

上海研卉生物科技有限公司

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上海研卉生物科技有限公司
李先生
18702156455 021-69000211
yanhuibio@163.com
产品属性
英文名DNA/RNA Shield
规格250ml
产品说明
ZYMO DNA/RNA Shield,DNA/RNA保存液
R1100-50DNA/RNA Shield50 ml
R1100-250DNA/RNA Shield250 ml
R1200-25DNA/RNA Shield (2X Concentrate)25 ml
R1200-125DNA/RNA Shield (2X Concentrate)125 ml
ZYMO DNA/RNA Shield-DNA/RNA保存试剂是用于任何生物样品中核酸的DNA和RNA稳定溶液。这种DNA和RNA稳定溶液在环境温度下保持了样本的遗传完整性和表达谱,并完全灭活了传染源(病毒、细菌、真菌和寄生虫)。独特的DNA和RNA稳定溶液还可以防止冻融循环和意外的冷冻失败导致的降解。
Applicable ForNext-Generation Sequencing, qPCR, microarray.
Device SpecsReagent
Reagent StorageAmbient temperature => 2 years
Sample Collection10% (v/v or w/v)
Sample SourceCells, tissues, blood, plasma, serum, saliva, urine, feces, environmental samples, etc.
Sample StabilityRNA: Ambient temperature (4°C- 25°C) >1 month
DNA: Ambient temperature (4°C- 25°C) >2 years
DNA & RNA: Frozen (<-20°C): Indefinitely
问题1:解冻保存在DNA/RNA保存液中的冷冻样品后出现白色沉淀,这是正常的吗?


在冻融过程中,试剂(或样品)的某些成分可能从溶液中沉淀出来。一旦样品解冻到环境温度,旋涡样品,使沉淀物回到溶液中。此外,试着用手或37°C加热样品5分钟,然后涡流。
问题2:在储存之前,我是否需要在Shield priorDNA/RNA 中对样品进行均质处理?


不,组织不需要均匀化。
问题3:如何在DNA/RNAShield中收集固体组织?


在室温下将组织转移到DNA-RNAShield层,浸泡。在组织匀浆之前,可以将组织匀浆或冷冻保存。

Q4: Can I FACS sort directly into DNA/RNA Shield?

Yes, sort directly into DNA/RNA Shield (e.g. Collect 100 ul of FACS sorted cells into minimally 400 ul of DNA/RNA Shield).

Q5: How long can samples be stored frozen in DNA/RNA Shield?

DNA & RNA (<-20°C): Indefinitely

Q6: How long can samples be stored samples in DNA/RNA Shield at ambient temperature?

RNA > 1 month DNA > 2 years

Q7: What sample types is DNA/RNA Shield suitable for?

DNA/RNA Shield can be used on a wide variety of sample types including cells, tissues, biological fluids (plasma, serum, saliva, urine), and environmental samples (feces, plants, swabs, etc.).

Q8: Can DNA/RNA Shield be used on extracted DNA and/or RNA?

Yes, extracted nucleic acids are stabilized in DNA/RNA Shield. Prior to downstream applications, clean-up the sample via the appropriate Zymo Research clean-up kit (Genomic DNA Clean and Concentrator (gDCC), DNA Clean and Concentrator (DCC), and RNA Clean and Concentrator (RCC)).