Isolate highly purified CD34+ cells from fresh whole umbilical cord blood (CB) using a simple, two-step procedure. First, hematopoietic progenitor cells are pre-enriched using the RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail (15226C) with antibodies recognizing T cell, B cell and myeloid cell surface markers. CD34+ cells are then selected using the EasySep™ Human CD34 Positive Selection Cocktail (18096C), which contains an antibody recognizing CD34.

This kit does not deplete platelets. If isolating CD34+ cells from whole CB in which platelet contamination is expected the EasySep™ Human Cord Blood CD34 Positive Selection Kit II (Catalog #17896) is recommended.

If isolating CD34+ cells from fresh blood or buffy coat, the Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086) is recommended.

If isolating CD34+ cells from other samples, including fresh or previously frozen mobilized peripheral blood or bone marrow mononuclear cells, or from previously frozen cord blood mononuclear cells, we recommend using the EasySep™ Human CD34 Positive Selection Kit (Catalog #18056).