重组人凝血因子8|Recombinant Human Coagulation Factor VIIIProSpec（蛋白质专家）是以色列一家具有超过16年历史的，专业从事细菌来源重组蛋白生产的先进生物技术公司。ProSpec生产多种细胞因子、生长因子、趋化因子、激素、酶、病毒抗原和许多其他重组蛋白。在研究团队和科学家们的努力下，ProSpec已经拥有超过1700种可用于癌症、细胞凋亡、发育、内分泌学、免疫学、神经科学、蛋白酶及干细胞研究用重组蛋白、多肽和抗体。10多年蛋白纯化方面的经验使ProSpec公司生产成本低、产量高、效率高，其蛋白产品不仅纯度高、生物活性高，价格也非常具有优势。同时ProSpec针对客户的需要提供自定义重组蛋白生产服务。 ProSpec向客户提供蛋白质产品、服务和技术授权。因为创新的产品、卓越的客户服务和对细节的注重，ProSpec在科学界建立了良好的信誉，具有广泛的客户群，包括世界各地的学术、政府研究机构，生物技术和制药企业以及医院和基准实验室。
产品详情：
CATALOGUE NUMBER
PRO-318
SYNONYMS
Coagulation factor VIII, Procoagulant component, Antihemophilic factor, AHF, F8, F8C, F8B, HEMA, FVIII, DXS1253E, F8 protein.
INTRODUCTION
Coagulation factor VIII participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder.
DESCRIPTION
Antihemophilic Facor Human Recombinant produced in CHO is a glycosylated polypeptide chain having a total amino acids of 1438 (170kd) and consisting of two dimer chains 80 kD and 90 kD.
The Factor-VIII lacks central region of B-domain region and is purified by proprietary chromatographic techniques.
SOURCE
CHO cells (Chinese Hamster Ovarian Cells).
PHYSICAL APPEARANCE
Sterile Filtered White lyophilized (freeze-dried) powder.
FORMULATION
Each IU was lyophilized from a solution containing 30ug PEG, 1.6ng VWF, 4ug calcium, and 0.25mg HSA.
SOLUBILITY
It is recommended to reconstitute 250IU lyophilized Factor-VIII in 5ml sterile 18M-cm H2O, which can then be further diluted to other aqueous solutions.
STABILITY
Lyophilized Factor-VIII although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Factor-VIII should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
PURITY
Greater than 97.0% as determined by SDS-PAGE.
BIOLOGICAL ACTIVITY
The specific activity was found to be 7,058 IU/mg.
USAGE
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
重组人凝血因子8|Recombinant Human Coagulation Factor VIII 
