产品货号 : jlcR4730 英文名称 : NADPH oxidase 4 中文名称 : NADPH氧化酶4抗体 别名 : Kidney oxidase-1; Kidney superoxide-producing NADPH oxidase; KOX 1; KOX; Kox-1; NADPH; NADPH oxidase 4; Nox4; NOX4_HUMAN; Renal NAD(P)H-oxidase; RENOX. 研究领域 : 肿瘤心血管细胞生物免疫学信号转导新陈代谢 抗体来源 : Rabbit 克隆类型 : Polyclonal 交叉反应 : Human, 产品应用 : WB=1:500-2000 ELISA=1:500-1000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. 分子量 : 67kDa 细胞定位 : 细胞膜 性状 : Lyophilized or Liquid 浓度 : 1mg/ml 免疫原 : KLH conjugated synthetic peptide derived from human NOX4:201-300/578 <Extracellular> 亚型 : IgG 纯化方法 : affinity purified by Protein A 储存液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. 保存条件 : Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. PubMed : PubMed 产品介绍 : Nox4 is a renal gp91-phox homolog highly expressed at the site of erythropoietin production in the proximal convoluted tubule epithelial cells of the renal cortex. Nox4 is also expressed in fetal tissues, placenta, glioblastoma and vascular cells. Like gp91-phox, the enzymatic activity of Nox4 produces superoxide anions. In vascular cells, the addition of angiotensin II increases Nox4 expression, which suggests a role for Nox-4 in vascular oxidative stress response.
Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 4 displays an increased activity. Isoform 5 and isoform 6 display reduced activity.
Subunit: Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4.
Subcellular Location: Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein (Probable). Cell junction, focal adhesion (Probable). Nucleus (Probable). Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
Tissue Specificity: Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.
Post-translational modifications: Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
