ELISA Kit for Albumin (ALB)
| Organism species | Oryctolagus cuniculus (Rabbit) |
| Product No. | CEB028Rb |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96-well strip plate |
| Assay length | 2.5 hours |
| Detection range | 617.28-50000ng/mL The standard curve concentrations used for the ELISA’s were 50000ng/mL, 16666.67ng/mL, 5555.56ng/mL, 1851.85ng/mL, 617.28ng/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 245.5ng/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Albumin (ALB).
No significant cross-reactivity or interference between Albumin (ALB) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Albumin (ALB) and the recovery rates were calculated by comparing the measured value to the expected amount of Albumin (ALB) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 88-103 | 99 |
| EDTA plasma(n=5) | 82-101 | 97 |
| heparin plasma(n=5) | 88-96 | 93 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Albumin (ALB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Albumin (ALB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Albumin (ALB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 96-105% | 79-103% | 91-105% | 85-101% |
| EDTA plasma(n=5) | 98-105% | 78-102% | 95-104% | 78-103% |
| heparin plasma(n=5) | 79-94% | 78-95% | 86-96% | 94-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Test principle
This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific for Albumin (ALB) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between Horseradish Peroxidase (HRP) labeled Albumin (ALB) and unlabeled Albumin (ALB) (Standards or samples) with the pre-coated antibody specific for Albumin (ALB). After incubation the unbound conjugate is washed off. The amount of bound HRP conjugate is reverse proportional to the concentration of Albumin (ALB) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Albumin (ALB) in the sample.
