| 免疫原 | E.coli-derived human Choline Acetyltransferase/CHAT recombinant protein (Position: T25-K731). |
| 亚型 | Rabbit IgG |
| 形态 | Lyophilized |
| 保存条件 | At -20℃ for one year. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for a longer time. Avoid repeated freezing and thawing. |
| 克隆性 | 单克隆 |
| 适应物种 | human,mouse,rat |
| 抗原来源 | Rabbit |
| 数量 | 现货 |
| 供应商 | 博士德生物 |
| 应用范围 | WB,IHC-P,ICC/IF,FCM,Direct ELISA |
| 浓度 | 500ug/ml |
| 规格 | 50ul/100ul/150ul |
| 货号 | A01192-4 |
|---|---|
| 产品名称 | ANTI-CHAT Antibody |
| 基因名 | CHAT |
| 抗体来源 | Rabbit |
| 克隆 | Polyclonal |
| 抗体亚型 | Rabbit IgG |
| 免疫原 | E.coli-derived human Choline Acetyltransferase/CHAT recombinant protein (Position: T25-K731). |
| 内容 | 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500ug/ml |
| 产品形态 | Lyophilized |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | Choline acetyltransferase (commonly abbreviated as°ChAT,but sometimes°CAT) is a transferase enzyme responsible for the synthesis of the neurotransmitter acetylcholine. In humans,the choline acetyltransferase enzyme is encoded by the°CHAT gene. This gene product is a characteristic feature of cholinergic neurons,and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene,and some of these variants have been shown to encode more than one isoform. |
| Uniprot ID | CHAT: P28329 |
| 推荐配套的二抗和检测试剂 | Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。 |
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
|---|---|
| Western blot: | 1:500-2000 |
| Immunohistochemistry in paraffin section: | 1:50-400 |
| Immunocytochemistry in fixed cells: | 1:50-400 |
| Flow Cytometry: | 1-3μg/1x106 cells |
| Direct ELISA： | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
