Product Description:

Lymphocyte Separation Media(LSM) is designed for vitro isolation of lymphocytes from whole flood. WISENT LSM is a sterile filtered solution at a density of 1.077-1.080g/mL. Endotoxin contained is below 0.06 EU/mL.

Instructions For Use:

1. LSM must be at room temperature.Thoroughly mix the LSM by inverting the bottle gently.

2. Aseptically transfer 3mL of LSM to a 15mL centrifuge tube.

3. Mix 2mL of blood with 2mL of PBS without Ca2+ and Mg2+.

4. Carefully layer diluted blood on top of the LSM,creating a sharp blood-LSM interphase. DO NOT MIX.

5. Aspirate the top layer of clear plasma to within 2-3mL above the lymphocyte layer and discard.

6. Aspirate the lymphocyte layer plus about half of the LSM layer into the centrifuge tube and centrifuge for 15 minutes at room temperature at a speed to sediment the cells without damage(400g). Washing the cells removes the LSM and reduces the percentage of platelets.

7. Wash the cells again with buffered balanced salt solution and resuspend in the appropriate medium for your applications.

Note:

1. For research use only.

2. Lymphocyte Separation Media(LSM) is designed for vitro isolation of lymphocytes from whole flood that

has been diluted and treated with anti-coagulant.

3. For the best results, use blood drawn less than 2 hours before the experiment. Do not use blood more than 24 hours after it was drawn.

FORM: Liquid, Sterile, Ready to use.

SHEIF LIFE: Maximum 18 months.

STORAGE TEMPERATURE: 2 to 8℃
热线：400-823-3718
网址：www.wisentbioproductschina.com