Application Notes:
PROTOCOL:
1. Invert the column several times to re-suspend the resin material. Remove the tip of the column and let the liquid drain off.
2. Buffer Equilibration: SpinOUT columns are supplied in deionized water. Equilibrate the column with a buffer of your choice, as follows. Note: Do not equilibrate the columns with Tris buffer. Apply about 0.5 mL of a desired buffer into the column. Let the buffer drain into collection tube. Repeat this process 3 times.
3. Place the column in a 2.0 mL micro-centrifuge tube. Centrifuge at 1000 xg for 20-30 seconds. Discard the liquid collected in the centrifuge tube.
4. Place the column back in the same centrifuge tube. Carefully apply the protein suspension to the center of the column. Wait for 1-2 minutes. Note: If your protein solution is in Tris buffer, exchange the protein solution buffer to phosphate or other buffer, using either a SpinOUT column or by dialysis.
5. After loading the column centrifuge at 1000 xg for 20-30 seconds. Collect the liquid comprising purified protein material free from detergent originally present in the sample.
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit or Assay Type:
Protein Extraction Kits
Components:
- Detergent-OUT Columns-DTG-100X 10 Micro
- Collection Tubes (2 mL) 10
Storage Conditions:
Store at 2-8°C for up to 6 months from date of purchase. To avoid possible microbial contamination, dispense all solutions aseptically.
Packaging:
10 columns
Reagent Type:
Detergents
Materials Required but Not Delivered:
Centrifuge
Centrifuge tubes
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详细描述见链接：http://www.millipore.com/catalogue/item/2114
