| 供应商 | 蓝景科信（北京）技术有限公司 |
| 规格 | 192 rxns |
The NEXTFLEX® Unique Dual Index Barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide unprecedented data security in sequencing applications. Multiplexing with NEXTFLEX® Unique Dual Index Barcodes drastically increases scale while reducing costs by allowing the user to pool multiple libraries in a single flow cell lane. These barcoded adapters can be used with single read or paired-end read sequencing, and are compatible with designed for Illumina® sequencing, or other workflows for Illumina® sequencing that involve the ligation of adapters to adenylated fragments.
Even users who don’t routinely perform high-throughput multiplexing benefit from the superior design of the NEXTFLEX® Unique Dual Index Barcodes. The ability to avoid using the same set of indices consecutively reduces concerns about run-to-run sample carryover and sample crosstalk. They are designed to specifically address the index hopping, or spread of signal, phenomenon associated with Illumina® platforms that utilize a patterned flow cell.
Cat # Name Quantity
NOVA-514150 NEXTFLEX® Unique Dual Index Barcodes 1-96 192 rxns
NOVA-514151 NEXTFLEX® Unique Dual Index Barcodes 97-192 192 rxns
NOVA-514152 NEXTFLEX® Unique Dual Index Barcodes 193-288 192 rxns
NOVA-514153 NEXTFLEX® Unique Dual Index Barcodes 289-384 192 rxns
2. NEXTFLEX-HT™ Barcodes
The NEXTFLEX-HT™ Barcodes are single-index adapters for Illumina® platforms that provide unprecedented flexibility and high-throughput capabilities in sequencing applications. Multiplexing with NEXTFLEX-HT™ Barcodes drastically increases scale while reducing costs, by allowing the user to pool multiple libraries in a single flow cell lane. These barcodes can be used with single read or paired-end read sequencing and are compatible with kits designed for Illumina® sequencing, and other Illumina® library preparation workflows that involve the ligation of adapters to adenylated fragments.
Cat # Name Quantity
NOVA-514170 NEXTflex-HT™ Barcodes - 6 (in tubes) 48 rxns
NOVA-514174 NEXTflex-HT™ Barcodes 1-96 (in plates) 192 rxns
NOVA-514175 NEXTflex-HT™ Barcodes 97-192 (in plates) 192 rxns
NOVA-514176 NEXTflex-HT™ Barcodes 193-288 (in plates) 192 rxns
NOVA-514177 NEXTflex-HT™ Barcodes 289-384 (in plates) 192 rxns
Custom NEXTflex-HT Barcodes 385 - 2,380 (in 96-well plate)
3. NEXTFLEX® DNA Barcodes
Cat # Name Quantity
NOVA-514101 NEXTflex™ DNA Barcodes - 6 48 rxns
NOVA-514102 NEXTflex™ DNA Barcodes - 12 96 rxns
NOVA-514103 NEXTflex™ DNA Barcodes - 24 192 rxns
NOVA-514104 NEXTflex™ DNA Barcodes - 48 384 rxns
NOVA-514105 NEXTflex-96™ DNA Barcodes（In plates） 768 rxns
NOVA-514106 NEXTflex-96™ DNA Barcodes（In tubes） 768 rxns
The NEXTFLEX-96™ DNA Barcodes are compatible with Illumina® sequencing and provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. These adapters compatible with Illumina®sequencing utilize an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads caused by single base errors introduced during PCR. The NEXTFLEX® index is contained within the adapter sequence, eliminating the need to perform PCR to add flow cell binding sequences. The NEXTFLEX-96™ DNA Barcode index sequences are designed to have a hamming distance of 4, allowing discrimination of different barcodes even in the case of an error in the barcode read.
These adapters are supplied in either a 96-well format (cat # NOVA-514105) or in microfuge tubes (cat # NOVA-514106). These barcodes can be used with single, paired-end, and multiplex reads and are compatible with the , the , the and other genomic DNA library prep protocols.
Cat # Name Quantity
NOVA-514105 NEXTflex-96™ DNA Barcodes-96 768 rxns
The NEXTFLEX® Dual-Indexed DNA Barcodes are two kits that each contain 96 combinatorial dual indexed barcoded adapters for Illumina® library prep which enable deep multiplexing of NGS sequencing samples compatible with high-throughput automation. They significantly increase scale, while reducing costs by allowing the user to pool up to 192 samples in a single flow cell.
Multiplexing Options
Libraries made using the NEXTFLEX® Dual-Indexed DNA Barcodes can be combined with single index libraries using a dual index sequencing read primer. The NEXTFLEX® Dual-Indexed DNA Barcodes can be used with single, paired-end and multiplex reads, and are compatible with the , the , the and other genomic DNA library prep kits.
Cat # Name Quantity
NOVA-514160 NEXTflex™ Dual-Indexed DNA Barcodes 768 rxns
NOVA-514161 NEXTflex™ Dual-Indexed DNA Barcodes 768 rxns
The NEXTFLEX® ChIP-Seq Barcodes are adapters containing indexed sequences that offer an improved multiplexing workflow and flexible setup. These barcodes are available in sets of 6, 12, 24, and 48 unique adapters. The ability to pool samples in an efficient way significantly decreases hands-on time while providing robust data quality. These barcodes can be used with single, paired-end, and multiplex reads. These adapters are compatible with the and the workflows.
Cat # Name QuantityThe NEXTFLEX-96™ ChIP-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX-96™ChIP-Seq Barcodes kits accomplish this by using an indexed adapter with an 8 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads caused by single base errors introduced during PCR. The NEXTFLEX® index is contained within the adapter sequence, eliminating the need to perform PCR to add flow cell binding sequences. The NEXTFLEX-96™ ChIP-Seq Barcode index sequences are designed to have a hamming distance of 4, allowing discrimination of different barcodes even in the case of an error in the barcode read. These adapters, compatible with Illumina®platforms, are available in custom formats.
Cat # Name Quantity
NOVA-514124 NEXTflex-96™ ChIP-seq Barcodes - 96 768 rxns
The NEXTFLEX® RNA-Seq Barcodes are indexed adapters that are designed for use with the and the . These indexes are composed of a 6 nt error-correcting sequence, offering an improved multiplex workflow and adding flexibility to library prep. The ability to pool samples in an efficient way significantly decreases hands on time while providing robust data quality. Their automation-friendly format enables multiplexing of up to 48 samples. The NEXTFLEX® RNA-Seq Barcodes are available in sets of 6, 12, 24 and 48 unique adapters. We also offer the, which contain 96 – 8 nt index barcodes in a 96-well format (cat # NOVA-512915) .
Cat # Name QuantityThe NEXTFLEX® Bisulfite-Seq Barcodes are designed to be used with the . Unlike other NEXTFLEX® Barcodes, the NEXTFLEX®Bisulfite-Seq Barcodes are methylated. The NEXTFLEX® Bisulfite-Seq Barcodes can be used to provide flexibility and high-throughput capabilities in sequencing applications. They significantly increase scale while reducing costs by allowing the user to pool multiple library preparations in a single flow cell lane. The NEXTFLEX® Bisulfite-Seq Barcodes kits accomplish this by using an indexed adapter with a 6 nt unique sequence. This allows for proper differentiation between samples, preventing poor reads from single base errors introduced during PCR.
These methylated adapters can be used with single, paired-end and multiplex reads.
Cat # Name Quantity
NOVA-511911 NEXTflex™ Bisulfite-Seq Barcodes - 6 48 rxns
NOVA-511912 NEXTflex™ Bisulfite-Seq Barcodes - 12 96 rxns
NOVA-511913 NEXTflex™ Bisulfite-Seq Barcodes - 24 192 rxns
