Fig1: ICC staining Phospho-AKT1(S124) in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig2: ICC staining Phospho-AKT1(S124) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining Phospho-AKT1(S124) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Phospho-AKT1(S124) antibody. Counter stained with hematoxylin.

Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-AKT1(S124) antibody. Counter stained with hematoxylin.