| 供应商 | 上海联迈生物工程有限公司 |
| 数量 | 大量 |
| 目录编号 | LM-0988R |
| 克隆性 | 多克隆 |
| 抗原来源 | Rabbit |
| 保质期 | 1年 |
| 抗体英文名 | CD71/Transferrin receptor |
| 抗体名 | 转铁蛋白受体抗体 |
| 宿主 | Rabbit |
| 适应物种 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, |
| 免疫原 | KLH conjugated synthetic peptide derived from human Transferrin receptor:621-720/760 <Extracellular> |
| 亚型 | IgG |
| 形态 | Lyophilized or Liquid |
| 应用范围 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /Test （石蜡切片需做抗原修复） |
| 浓度 | 1mg/ml |
| 保存条件 | Store at -20 °C |
| 规格 | 50ul 100ul 200ul |
| 英文名称 | CD71/Transferrin receptor |
| 中文名称 | 转铁蛋白受体抗体 |
| 别 名 | CD 71; CD71; CD71 antigen; p90; T9; TFR 1; TFR; TFR1; TFRC; TR; Transferrin receptor (p90 CD71); Transferrin Receptor Protein 1; OTTHUMP00000208523; OTTHUMP00000208524; OTTHUMP00000208525; Transferrin receptor protein 1, serum form; TRFR. |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 心血管 细胞生物 免疫学 信号转导 干细胞 转录调节因子 细胞表面分子 细胞类型标志物 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, Horse, Guinea Pig, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 Flow-Cyt=1μg /Test （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 74/85kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Transferrin receptor:621-720/760 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Cellular uptake of iron occurs via receptor mediated endocytosis of ligand occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Useful in studies of dividing haematopoietic and tumour cell populations, and metabolic activity. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C terminal binding site. The antigen is present on most dividing cells, including normally cycling in vivo hematopoietic progenitor cells, mitogenically stimulated cells in vitro, some primary tumor cells and most proliferating cells in vitro. Function: Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Subunit: Homodimer; disulfide-linked. Binds one transferrin molecule per subunit. Interacts with SH3BP4. Subcellular Location: Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Post-translational modifications: N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. Similarity: Belongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain. SWISS: P02786 Gene ID: 7037 Database links: Entrez Gene: 7037 Human Entrez Gene: 22042 Mouse Entrez Gene: 64678 Rat Omim: 190010 Human SwissProt: P02786 Human SwissProt: Q62351 Mouse SwissProt: Q99376 Rat Unigene: 529618 Human Unigene: 28683 Mouse Unigene: 98672 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 转铁蛋白及转铁蛋白受体(TfnrR)是机体铁代谢中的重要因子，铁是细胞生长增殖、代谢的必需元素,转铁蛋白受体(TfR)介导的胞吞作用是细胞摄取铁的主要途径。肿瘤细胞因增殖生长旺盛,对铁的需求高,所以肿瘤细胞有比正常细胞更多的转铁蛋白受体的表达。 |
| 产品图片 |  Sample: Placenta (Mouse) Lysate at 40 ug Primary: Anti-CD71/Transferrin receptor (bs-0988R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74/85 kD Observed band size: 75 kD  Blank control(blue):Raji(fixed with 2% paraformaldehyde (10 min)). Primary Antibody:Rabbit Anti- CD71 antibody(bs-0988R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. |
