Cat code200947-2E1

Species Cross-reactivity H,M,Mk,R,Hm

Key Application WB,IP,ICC,IHC

HostMouse

Clone Number2E1-G10-H10

Antibody type Monoclonal antibody

Purified method

Isotype IgG2b

Molecular Weight 36kDa

Sensitivity This antibody detects endogenous levels of PCNA and does not cross-react with related proteins.

Immunogen Purified recombinant human PCNA protein fragments expressed in E.coli.

UniProt Number P12004

GeneBank ID NM_002592

Subcellular location Nucleus.

Formulation ascites

Alternate names Cyclin;DNA polymerase delta auxiliary protein;HGCN8729;MGC8367;Mutagen-sensitive 209 protein;OTTHUMP00000030189;OTTHUMP00000030190;PCNA;Pcna/cyclin;PCNA_HUMAN;PCNAR;Polymerase delta accessory protein;Proliferating Cell Nuclear Antigen.

Gene_symbol

Summary The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta.The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.In response to DNA damage,this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.Two transcript variants encoding the same protein have been found for this gene.Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.[provided by RefSeq,Jul 2008]

UniPort summary Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase´s processibility during elongation of the leading strand.Induces a robust stimulatory effect on the 3´-5´ exonuclease and 3´-phosphodiesterase,but not apurinic-apyrimidinic (AP) endonuclease,APEX2 activities.Has to be loaded onto DNA in order to be able to stimulate APEX2.Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways.Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair:Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases,while ´Lys-63´-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Application Images :

Western blot detection of PCNA in Hela,3T3,COS7,C6 and CHO-K1 cell lysates using PCNA mouse mAb (1:1000 diluted).Predicted band size:36KDa.Observed band size:36KDa.

Immunocytochemistry staining of HeLa cells using PCNA mouse mAb (dilution 1:100).Fixed in 100% methanol for 2hr at -20℃.

Immunoprecipitation analysis of Hela cell lysates using PCNA mouse mAb.

Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with PCNA mouse mAb (2E1-G10-H10,1:400 diluted),showing nuclear localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).