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产品名称：TUNEL原位细胞凋亡检测试剂盒(AP)
英文名称：In Situ Cell Apoptosis Detection Kit, AP
产品货号：GS0249
产品规格：20次|100次

储存条件：冷冻（-20℃）

概述

Apoptosis is essential in many physiological processes.Several methods have been described to identify apoptotic cells.Endonucleolysis is considered as the key biochemical event of apoptosis,resulting in double-stranded,low molecular weight DNA fragments as well as single strand breaks in high molecular weight DNA.Those DNA strand breaks can be identified by labeling free 3’-OH termini with modified nucleotides in an enzymatic reaction.Terminal deoxynucleotidyl transferase(TdT)can catalyze DIG-dUTP to free 3’-OH DNA ends.The DIG-dUTP is first react with biotin-conjugated anti-digoxin antibody,which subsequently associate with streptavidin-AP(SABC-AP).Combined with BCIP/NBT Chromogenic Reagent,apoptotic cells stain Purple-blue analyzed under microscopy.

组份

Component A:Labeling Buffer;Component B:TdT(20X);Component C:DIG-dUTP(20X);Component D:Blocking Reagent;Component E:Biotin-conjugated anti-digoxin antibody;Component F:SABC-AP(100X);Component G:Protein K(200X);Component H:BCIP/NBT Chromogenic Reagent(20X);Component I:Antibody Dilution;Positive Control



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