Product Description

Mesenchymal stem cells (MSCs) are believed to reside in bone marrow perivascular niches where they create and function as a stromal network, supporting other cell types and contributing to the creation and maintenance of connective tissues. These cells have long been believed to be multipotent stromal cells that can differentiate into the cells of the connective tissue in vitro, such as chondrocytes (cartilage), osteoblasts (bone), and adipocytes (fat). Although little is known of the phenotypic characteristics of MSCs in vivo, MSCs in culture are defined by the expression of CD73, CD90, and CD105 and the lack of CD11b, CD19, CD34, CD45, and HLA DR. Cultured MSCs have shown promising applications in cell-based therapeutic strategies for specific tissues.

Bone marrow mononuclear cells are cultured for 9~14 days using the MesenCult™ ACF Culture Kit. After culture adherent cells are checked for the expression of CD73, CD90, and CD105 and the lack of CD14, CD34, and CD45 expression by flow cytometry.

Cells were obtained using Institutional Review Board (IRB) approved consent forms and protocols.

Product Specification

Flow Data

Figure 1. Representative flow data of enriched MSCs from bone marrow prior to cryopreservation.MSCs have a ≥90% expression of CD90, CD105, and CD73.

Figure 2. Representative flow data of enriched MSCs from bone marrow prior to cryopreservation.MSCs have <5% expression of CD14, CD34, and CD45 (panel of antibodies used).