HepG2.2.15细胞室：人肝癌细胞株 货号：GD-C3218883
细胞来源：细胞主要来源ATCC、DSMZ、ECACC、RIKEN、INCell、promocell、ScienCell、ECACC、JCRB、KCLB、Asterand、ICLC以及少数国外著名大学建系
产品包装：复苏形式：T25培养瓶(一瓶)或冻存形式：1ml冻存管(两支)
产品规格：≥1*10(6)/ml
安全级别：1
传代培养及其操作步骤：原代细胞培养成功以后，需要进行分离培养，否则细胞会因生存空间不足或密度过大，营养障碍，影响细胞生长。细胞由原培养瓶内分离稀释后传到新的培养瓶中培养的过程称之为传代培养。传代细胞允许培养的细胞扩增(形成细胞株)，可以进行细胞克隆，易于保存，但可能丧失一些特殊的细胞和分化特征。传代细胞形成细胞株的最大利处在于提供了大量持久的实验材料，便于实验。传代细胞培养操作步骤：1)吸掉或倒掉培养瓶内旧培养液；2)向瓶内加入胰蛋白酶液和EDTA混合液少量。以能覆盖培养瓶底为宜；3)置37℃孵箱或室温(25℃温度)下进行消化，2-5min后把培养瓶放在倒置显微镜下进行观察，当发现胞质回缩、细胞间隙增大后，应立即中止消化；4)吸出消化液，向瓶内加入Hanks液少量，轻轻转动培养瓶，把残留消化液冲掉，然后再加培养液。如果仅使用胰蛋白酶消化，在吸除胰蛋白液后，可直接加入少量含血清的培养液，终止消化；5)使用弯头吸管，吸取瓶内培养液，按顺序反复轻轻吹打瓶壁细胞，使之从瓶壁脱离形成细胞悬液。吹打时动作要轻柔，以防用力过猛损伤细胞；6)用计数板计数后，分别接种于新的培养瓶中，置CO2培养箱中进行培养；7)细胞培养换液时间应根据细胞生长的状态和实验要求来确定。一般2-3d后应换一次生长液。待细胞铺满器皿底面，即可使用；也可继续传代扩大培养或换成维持液。
物种来源：人源或鼠源等
细胞形态：上皮细胞样/成纤维细胞样/淋巴母细胞样等
细胞特性：贴壁/悬浮等
细胞检测：细胞不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌等污染物
培养条件：详见细胞说明书
传代方法：建议1:2-1:3传代；两天换液一次(具体详细询问公司细胞培养专家)
【细胞培养中原虫的污染情况总结】原虫：培养液可轻微浑浊，显微镜下那些细小的点状物数量非常多，轻微活动，细胞虽然可以生长但繁殖速度却明显减慢，而且细胞状态不好，边缘不清楚，细胞不透亮。他们与细胞可共生但会与细胞争夺营养。这种共生是非常普遍的，但他们的数量小，细胞站优势所以不会影响到细胞的正常生长，只有当他们到达一定的数量时就会影响到细胞的生长，最终形成恶性循环。污染的可能原因：可能原因很多比如配液消毒问题、操作问题、环境问题等等关于培养基的无菌状况，取培养基至培养瓶中(不加细胞)，37度试培养一段时间后观察。如果没有细菌生长就是操作的问题。也可以在培养基中事先加入双抗(硫酸链霉素和氨苄青霉素)。但双抗有时会影响细胞的状态，所以在做转染、检测细胞某项指标前一定要撤去双抗，以避免影响实验结果。1)孵箱应定期用三氧机消毒或者紫外光照射，并用酒精和新洁尔灭试擦孵箱同时孵箱内的水应是三蒸水；2)超净台\取材\器材\培养液\培养瓶\操作等因素；3)超净台的风机不能过大，风机到6-8格。否则也可能能致霉菌污染；4)无菌室经甲醛熏蒸消毒后，可用同等量的氨水喷洒中和，约几小时即可进入操作。
DL-^b-Proline hydrochloride, 95%
4-Fluoro-2-(trifluoroMethyl)benzonitrile
9-HYDROXY-4-METHOXYACRIDINE
Diphenylphosphine oxide
3-(4-Methoxyphenyl)propan-1-ol
BTS
Methyl 4-chloro-3-nitrobenzoate
Dihexyl Phthalate
4-Bromo-2-methoxyaniline 99%
4-(4,4,5,5-Tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl acetate, min. 97%
Boc-2-methyl-L-phenylalanine
2-IODOBENZOPHENONE
ETHYL 5-AMINO-1-METHYLPYRAZOLE-4-CARBOXYLATE
3-CHLORO-2,4-DIFLUORONITROBENZENE
4-Aminophenylacetonitrile
7-BroMo-1-benzofuran
Methyl 2,5-Dihydrothiophene-3-carboxylate
N-Cyclohexyl-N-Methyl 2-broMobenzaMide
ThalliuM(III) nitrate trihydrate, 99.5%
Ofloxacin
3-Nitrobenzotrifluoride
8-[5-(5,7-dihydroxy-4-oxo-4H-chromen-2-yl)-2-methoxyphenyl]-...
1-(2,6-dichlorobenzyl-ideneamino)-*guanidine
Phenyldimethylsilane
(-)-Episesamin
Propyl gallate
L-Methionine methyl ester hydrochloride
DL-Menthol
5-BroMo-4-fluoro-1,3-benzodioxole, 96%
4,5,6,7-Tetrahydrobenzo[b]thiophene-2-carboxylic acid, 97%
6-Chloro-3-iodo-4H-chroMen-4-one, 97%
Andrographolide
Dichlorotetrakis[2-(2-pyridyl)phenyl]diiridiuM(III)
sec-Butyl disulfide
5-METHYL-1-PHENYL-1H-PYRAZOLE-4-CARBOXYLIC ACID
3,4-Difluoro-2-Methoxyaniline sulfate
4-Methoxy-3-(trifluoromethyl)phenylboronic acid
3'-Iodoacetophenone
N-(Carbamothioyl)benzamide
Ethyl 6-(chloromethyl)pyridine-2-carboxylate ,97%
4,10-Diaza-15-crown 5-Ether
2-Biphenylcarboxaldehyde
Synephrine tartrate
Propyl chloroformate
Ethyl 2-Methyl-1,3-dioxolane-2-acetate
2,3,5,6-Tetrafluorophenol
Di-Mu-chlorobis(2'-aMino-1,1'-biphenyl-2-yl-C,N)dipalladiuM(II)
N-tert-Butyl 3-broMobenzenesulfonaMide
tert-Butyl 4-(Chlorosulfonyl)piperidine-1-carboxylate, 97%
Tropaeolin O
2-Ethyl-4-MethyliMidazole
dimethyl chlorophosphate
2,3-dihydro-1,4-benzodioxine-6-carbonyl chloride
Sulphur Brown 4
4-(2-dimethylaminoethyl)phenol
Trichloropropylsilane
Triphenylphosphine-3,3',3''-trisulfonic acid trisodiuM salt
2,3,4-TRIFLUOROPHENYL ISOCYANATE
Octadecy triMethyl aMMoniuM broMide
(R,R)-(-)-2,5-Bis(methoxymethyl)pyrrolidine, 96%
Methyl 4-bromo-1-benzothiophene-2-carboxylate
(R)-5-Allyl-2-oxabicyclo[3.3.0]oct-8-ene
Isothiazole-5-carboxylic acid
Sodium molybdate dihydrate
1H,1H,12H,12H-PERFLUORO-1,12-DODECANEDIOL
3-Chloropropyl methyl ether
Ruthenium(IV) oxide hydrate
2-AMino-5-broMobenzoic Acid
1,3-AdaMantanediacetic Acid
1-tert-Butyl-2-azetidinecarboxylic acid
4-(AMinoMethyl)benzoic acid, 99%
2-ChloropyriMidine, 98+%
DIALLYL DICARBONATE
SodiuM SulfaMate
1-Tetradecanol
(R)-2-(Boc-amino)-2-phenylethylamine
N(alpha)-fmoc-S-tert-butyl-L-cysteine
4-Propylcyclohexanone
Porcine angiotension Ⅱ,ANG-Ⅱ ELISA Kit
Mouse Interferon γ ,IFN-γ ELISA Kit
Human LIPN ELISA Kit
Human CAPN10 ELISA Kit
NRG1 ELISA Kit
CIV ELISA Kit
E2 ELISA Kit
Human PD-1 ELISA Kit
Human TD-Ag ELISA Kit
DNASE1 ELISA Kit
Malt Extract Broth
ISP Medium4(Inorganic Salts-Starch Agar)
Indole-3-Acetic Acid
PiMelic Acid
5-AMinoisophthalic acid, 95%
2-METHYL-2-PENTENAL
8-[5-(5,7-dihydroxy-4-oxo-4H-chromen-2-yl)-2-methoxyphenyl]-...
NITROGEN PENTOXIDE
2-Amino-3-formylpyridine
1,3-Dimethoxy benzene

METHYL 4-AMINO-3-CHLOROBENZOATE
(-)-GALLOCATECHIN
Boc-N-Methyl-D-Alanine
2-Methyl-4-nitrobenzoic acid, 97%
3-BroMopyridine, 98+%
2-AMino-4,5-diMethoxybenzonitrile
N-Methyl-1-naphthylMethylaMine Hydrochloride
2,2-DIHYDROXY-2,2-DIISOBUTYLPROPANE
Methyl 6-Methylnicotinate
1,3-DibroMo-2,2-diMethoxypropane
Ethyl 4-hydroxy-8-Methylquinoline-3-carboxylate
2,6-Difluoro-4-hydroxybenzeneboronic acid
Strontium oxide (99.9%-Sr)
N-(6-Aminohexyl)-5-chloronaphthalene-1-sulphonamide hydrochloride
4,5,6,7-Tetraiodoisobenzofuran-1,3-dione