生物芯片、基因芯片、细胞芯片Epoxysilane coating

价格：¥120

品牌：SCHOTT

最新更新日期：2021-08-17 03:42

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广州芯奥光电科技有限公司

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公司：广州芯奥光电科技有限公司

联系人：曾先生

联系电话： 18566239007 020-82510371

联系邮箱：don.zeng@siooptics.com

QQ： 76550682

产品说明

Epoxysilane coating

NEXTERION^® Slide E

NEXTERION^® Slide E is the market leading epoxy coated slide from SCHOTT. The slides are manufactured from high quality borosilicate glass that has an ultra flat surface and low inherent fluorescence. The glass is coated with a multi-purpose epoxysilane layer that will covalently bind most types of bio-molecules including amino- and non-modified DNA, RNA, and proteins. The defect-free surface features a uniform epoxysilane layer that provides a high covalent coupling efficiency together with a very low background. The slides are easy to use, and are fully compatible with all commercially available arraying and scanning instruments.

Overview

Type of coating	Immobilization method	Typical probes	Ordering information
			NEXTERION^®product	Barcode option	Item number	Slides per pack
Epoxysilane 2-D surface	Amino-, thiol- and hydroxylreactive chemistry Covalent binding	• Amino-modified or unmodified oligos, mRNA, PCR and BACs • Proteins	Slide E	None	1066643	25
				Laser	1064016	25

Key product features

• Covalent and directed binding of DNA and proteins

• Multi-purpose microarray slide coating

• Hydrophobic coating enables the spotting of high-density arrays

• Stability of the epoxy chemistry supports long print runs

Typical applications

• Transcriptional profiling

• MicroRNA

• Array-CGH

• SNP detection

• ZIP-code universal microarray

• Pathogen identification

• Subtelomere arrays

• Functional protein arrays

• Protein profiling arrays

Suitable probe types

• Amino-modified oligonucleotides 20 – 70 mers

• Unmodified oligonucleotides 20 – 70 mers

• Amino-modified PCR products

• ZIP-code oligonucleotides

• PCR products

• BAC/PACs

• L-DNA

• cDNA

• RNA

• Serum samples

• Antibodies

• Peptides

• Glycans

Immobilization chemistry

The diagram shows a schematic representation of an amino-modified DNA molecule coupling to NEXTERION^®Slide E. Amino-modified nucleic acids bind via the terminal amino group, as well as via exocyclic amino groups of the bases, and hydroxyl groups of the sugar-phosphate backbone. Unmodified nucleic acids will bind to an epoxysilane surface with almost the same efficiency via the exocyclic amino groups of the bases, and hydroxyl groups of the backbone. Other bio-molecules such as proteins bind via their exposed amine-, thiol- and hydroxyl-groups. The reaction with the epoxy group is rapid and irreversible and no additional baking or UV cross-linking steps are required. Any unreacted epoxy group may be chemically deactivated after printing to significantly reduce nonspecific binding of the target molecules to the slide surface. Molecular spacers between the glass slide and the epoxy groups facilitate interactions between the printed bio-molecules and their binding partners in solution.

Highly reproducible coating
NEXTERION^® Slide E is produced using an innovative proprietary process developed by SCHOTT to produce a uniform and reproducible epoxysilane coating on both sides of a high quality borosilicate glass slide. All slides are individually examined for physical defects and the presence of particles before and after coating. The epoxysilane surface is applied in tightly controlled, class 100 clean room facility, resulting in coated slides with highly uniform surface properties and low auto-fluorescence.

Fully optimized surface

NEXTERION^® Slide E provides excellent spot morphologies and reproducible spot sizes with a variety of commonly used spotting buffers. The surface chemistry is robust and stable, and remains active even during very long print runs. The density of the epoxy groups in the coating is constant over the entire surface of the slides, and has been optimized to provide uniform spot size and shape and the optimal binding capacity. Another important parameter, surface hydrophobicity, is also tightly controlled to optimize the performance of the slides with both contact and non-contact printers.

Packaging and storage

NEXTERION^® Slide E are packaged in chemically stable plastic boxes and sealed under an inert atmosphere. The slides are ready-to-use from the box, and are stable for 12 months in the sealed packaging when stored at room temperature.

Format

The slides are available in packs of 25 slides with optional code 128 barcodes enabling automated sample tracking. The identical epoxysilane coating is also available in 16-well slide, 48-well slide and 96-well microplate multi-well formats. For further information refer to the section on “Multi-well formats”.

Comaptible reagents for printing DNA and Oligos

Protocol step	Recommended NEXTERION^®products	Alternatives	Remarks
Spotting	NEXTERION^®Spot NEXTERION^®Spot LE Kit (low evaporation buffer)	NEXTERION^® Spot + detergents like Cetyltrimethylammoniumbromide, Triton X-100, sarcosyl, Tween or SDS with a final concentration between 0.005 and 0.05 % (to increase the spot size) 3x SSC	Recommended Concentrations: Oligonucleotides: 10 – 20 µM PCR products: 0.1 – 0.5 µg/µL
Chemical deactivation of unreacted Epoxy groups	NEXTERION^®Block E	50 mM ethanolamine + 0.1 % SDS in 0.1 M Tris, pH 9.0
Chemical deactivation of unreacted Epoxy groups	NEXTERION^®Oligo Pre-Hyb
Hybridization	NEXTERION^®Hyb	3 – 5x SSC + 0.1 % SDS	No formamide
Hybridization	NEXTERION^®Oligo Hyb		Contains formamide
Washing steps	NEXTERION^®Wash A and B

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