Cell Meter 钙检测流式细胞仪分析检测试剂盒

¥6240
AAT Bioquest
进口
2021-08-17 04:30

上海齐源生物科技有限公司

我要认领
上海齐源生物科技有限公司
杨阳
15021410950
3123991360@qq.com
产品属性
数量大量
英文名Cell Meter™ Flow Cytometric Calcium Assay Kit
CAS号/
保质期详见说明
供应商齐源生物
保存条件详见说明
规格100 Tests
产品说明
齐源生物专业代理美国AAT Bioquest产品,并有大量储存现货欢迎致电:021-59588506 
关键词:Cell Meter 钙检测流式细胞仪分析检测试剂盒详细如下
Cell Meter™ Flow Cytometric Calcium Assay Kit provides fluorescence-based assays for detecting intracellular calcium mobilization using a flow cytometer. It can be used for kinetic reading or for endpoint reading. After loading the Calbryte™ 520 AM dye into cells of interest, simply wash the cells and add the calcium flux agonist, one can then read the sample via a flow cytometer using kinetic reading mode or endpoint reading mode. Calbryte™ 520 AM can cross cell membrane passively by diffusion. Once inside the cells, the lipophilic blocking groups of Calbryte™ 520 AM are cleaved by esterase, resulting in a negatively charged fluorescent dye that stays inside cells. Its fluorescence is greatly enhanced upon binding to calcium. When cells expressing GPCR of interest are stimulated with an agonist, the receptor signals the release of intracellular calcium, which significantly increases the fluorescence of Calbryte™ 520. The Cell Meter™ Flow Cytometric Calcium Assay Kit can be used for monitoring cellular calcium flux as well as cell sorting
Components
Component A: Calbryte™ 520 AM 1 vial, lyophilized
Component B: Assay Buffer 1 bottle (50 mL)
Component C: Probenecid (Optional) 1 bottle (3 mL, 25mM)
Component D: HHBS (Hanks’ with 20 mM Hepes) 1 bottle (100 mL)
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