Blood vessels of the skin are composed of a number of cellular and non-cellular elements.
Thepredominant cell type in all skin blood vessels is the endothelial cell. Endothelial cells form the
interface between intravascular and extravascular compartments and serve as a selective barrier for
the diffusion of cells and macromolecules between these two compartments. The endothelial cells
lining blood vessels of the skin share features of endothelial cells elsewhere in the body, but they also
exhibit several skin specific characteristics. They are active participants in a variety of physiological
processes. These include wound healing, control of hemostasis, temperature regulation, and the
modulation of inflammation/leukocyte trafficking [1, 2]. The structure and function of endothelial cells
are differentially regulated in space and time [3]. Endothelial cell proliferation, quiescence, apoptosis,
and senescence are the four principal cytologic states that set the cutaneous microvascular in a
dynamic balance between maintenance and remodeling [4].
HDMEC-a from ScienCell Research Laboratories are isolated from human dermal tissue. HDMEC-a are
cryopreserved on passage one and delivered frozen. Each vial contains >5 x 105 cells in 1 ml volume.
HDMEC-a are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and
CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HDMEC-a are negative for HIV-1, HBV, HCV, mycoplasma,
bacteria, yeast and fungi. HDMEC-a are guaranteed to further expand for 15 population doublings in
the conditions provided by ScienCell Research Laboratories.
Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HDMEC-a.
Product Use
HDMEC-a are for research use only. It is not approved for human or animal use, or for application in in
vitro diagnostic procedures.
Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells
in liquid nitrogen until cell culture needed for experiments
Shipping
Dry ice.
Reference
[1] Swerlick, R. A and Lawley, T. J. (1993) Role of microvascular endothelial cells in inflammation. J. Invest.
Dermatol. 100:111S.
[2] Springer, T. (1994) Traffic signals for lymphocyte recirculation and leukocyte emigration. Cell 76:301.
[3] Aird, W. C. (2003) Endothelial cell heterogeneity. Crit. Care Med. 31(4):S221-30.
Sciencell产品目录:
|
| 真皮细胞系统 |
| 中文名 | 货号 | 简称 | 英文名称 |
| 人真皮微血管内皮细胞 | 2000 | HDMEC | Human Dermal Microvascular Endothelial Cells |
| 人真皮淋巴内皮细胞 | 2010 | HDLEC | Human Dermal Lymphatic Endothelial Cells |
| 人真皮微血管内皮细胞-成人 | 2020 | HDMEC-a | Human Dermal Microvascular Endothelial Cells-adult |
| 人表皮角质形成细胞-新生儿 | 2100 | HEK | Human Epidermal Keratinocytes-neonatal |
| 人表皮角质形成细胞- 成人 | 2110 | HEK-a | Human Epidermal Keratinocytes-adult |
| 人表皮角质形成细胞- 胎儿 | 2120 | HEK-f | Human Epidermal Keratinocytes-fetal |
| 人表皮黑色素细胞--浅色素 | 2200 | HEM-l | Human Epidermal Melanocytes-light |
| 人表皮黑色素细胞--中色素 | 2210 | HEM-m | Human Epidermal Melanocytes-medium |
| 人表皮黑色素细胞--深色素 | 2220 | HEM-d | Human Epidermal Melanocytes-dark |
| 人表皮黑素细胞-成人 | 2230 | HEM-a | Human Epidermal Melanocyte-adult |
| 人真皮成纤维细胞-胎儿 | 2300 | HDF-f | Human Dermal Fibroblasts-fetal |
| 人真皮成纤维细胞- 新生儿 | 2310 | HDF-n | Human Dermal Fibroblasts-neonatal |
| 人真皮成纤维细胞- 成人 | 2320 | HDF-a | Human Dermal Fibroblasts-adult |
| 人真皮成纤维细胞-胎儿-丝裂霉素处理 | 2350 | HDF-f-mt | Human Dermal Fibroblasts-Fetal-Mitomycin C treated |
| | | |
| 毛发细胞系统 | |
| 中文名 | 货号 | 简称 | 英文名称 |
| 人毛乳头细胞 | 2400 | HHDPC | Human Hair Dermal Papilla Cells |
| 人生发基质细胞 | 2410 | HHGMC | Human Hair Germinal Matrix Cells |
| 人毛囊外根鞘细胞 | 2420 | HHORSC | Human Hair Outer Root Sheath Cells |
| 人毛囊内根鞘细胞 | 2430 | HHIRSC | Human Hair Inner Root Sheath Cells |
| 人毛囊角质细胞 | 2440 | HHFK | Human Hair Follicular Keratinocytes |
| | | |
|
| 大鼠细胞系统 |
| 中文名 | 货号 | 简称 | 英文名称 |
| | | |
| 大鼠表皮角质形成细胞-新生大鼠 | R2100 | REK-n | Rat Epidermal Keratinocytes-neonate |
| 大鼠真皮成纤维细胞 | R2300 | RDF-n | Rat Dermal Fibroblasts |
| 大鼠真皮成纤维细胞- 成人 | R2320 | RDF-a | Rat Dermal Fibroblasts-adult |
| | | |
