Abnova产品货号： CO0002
产品全称：  RNA (-) 对照 CISH(显色原位杂交技术)探针
- 【RNA (-) Control CISH Probe】
产品描述：RNA( - )对照 CISH探针设计用于通过显色原位杂交技术(CISH)评估福尔马林固定,石蜡包埋的组织或细胞中的非特异性背景染色.
- 【RNA (-) Control CISH Probe is designed to be used for assessing the unspecific background staining in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).】
基因名称及描述： 
暂无说明
抗原种属： 暂无说明
适用实验样本种属： 暂无说明
承诺应用实验类型：CISH-P
注意事项： 该探针旨在与CISH实施HRP-DAB试剂盒结合使用(目录编号: KA5367 ),为样品预处理和杂交后处理提供必要的试剂.
该探针由一组随机序列寡核苷酸组成,GC含量为40-70％,未达成共识任何天然存在的序列.该探针不应产生阳性染色信号,并用于评估标本内的非特异性背景染色.信号的可视化应通过光学显微镜使用10x或20x物镜进行.对于信号评估,应避免坏死,退化或过度消化的细胞,因为这些细胞经常非特异性染色.为了判断杂交信号的特异性并确认该方法的正确性能,任何杂交都应伴随对照.我们建议使用至少一个含有真阳性和阴性染色细胞的对照样品.
- 【The probe is intended to be used in combination with the CISH Implementation HRP-DAB Kit (Catalog #: KA5367), which provides necessary reagents for specimen pretreatment and post-hybridization processing. This probe consists of a set of random sequence oligonucleotides with GC contents of 40-70% without known consensus to any naturally occurring sequences. This probe should not result in positive staining signals and is to be used to assess the unspecific background staining within specimens. Visualization of signals should be performed by light microscopy using a 10x or 20x objective. For signal evaluation, necrotic, degenerated or over-digested cells should be avoided as these cells often stain nonspecifically. In order to judge the specificity of the hybridization signals and to confirm the correct performance of the method, any hybridization should be accompanied by controls. We recommend using at least one control sample containing both true positive and negative staining cells. 】