【产品介绍】癌基因蛋白C-myc是一种位于 细胞核内的转录因子，它由439个氨基酸组成，参与细胞的增殖、分化和凋亡等一系列过程。C－myc蛋白表面一共有7个磷酸化位点，其中2个是苏氨酸磷酸 化位点，5个是丝氨酸磷酸化位点。研究表面，在胃癌，乳腺癌，结肠癌和宫颈癌等许多肿瘤组织或细胞中，C－myc蛋白的表达量要明显高于正常的组织和细胞 中的表达量。
C-myc，c-MYC抗体
名 称:
c-MYC 抗体
货 号:
AF0358
来 源:
Rabbit
应 用:
WB,IHC,IF/ICC,ELISA
反 应:
Human, Mouse, Rat
预 测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
蛋白号:
P01106
分子量:
49kDa
浓 度:
1mg/ml
Applications:
WB: 1:500-1:3000, IHC: 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Reference Citations:
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Upregulation of E‑cadherin expression mediated by a novel dsRNA suppresses the growth and metastasis of bladder cancer cells by inhibiting β-catenin/TCF target genes.Int J Oncol 2018 Jun;52(6):1815-1826
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2). Tang H et al.
Neoisoliquiritigenin Inhibits Tumor Progression by Targeting GRP78-β- catenin Signaling in Breast Cancer.Curr Cancer Drug Targets 2018;18(4):390-399
(PubMed: 28914191)
3). Luo S et al.
Sphingomyelin synthase 2 overexpression promotes cisplatin-induced apoptosis of HepG2 cells.Oncol Lett 2018 Jan;15(1):483-488
(PubMed: 29375716)
4). Zhang Q et al.
Enhancing E-cadherin expression via promoter-targeted miR-373 suppresses bladder cancer cells growth and metastasis.Oncotarget 2017 Sep 30;8(55):93969-93983
(PubMed: 29212202)
5). Tong Lu et al. Role of Wnt/β-catenin signaling pathway in the repair of intestinal mucosa associated with crypt stem cell in a rat model of abdominal compartment syndrome.Int J Clin Exp Pathol 2017;10(2):2351-2362
6). et al. Fungal Proteins from Hericium Erinaceus Show Auxiliary Antitumor Effects with 5-Fluoro-2,4(1H,3H)-Pyrimidinedione by Improving the Gut Microbiota in Mice.
7). et al. Fungal Proteins from Hericium Erinaceus Show Auxiliary Antitumor Effects with 5-Fluoro-2, 4 (1H, 3H)-Pyrimidinedione by Improving the Gut Microbiota in Mice.
Western blot analysis on 293 cell lysates using MYC Antibody,The lane on the left was treated with the antigen-specific peptide.
AF0358 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
AF0358 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
