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限制性内切酶

¥1930
Thermo Scientific
原装进口
2021-08-25 10:56
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上海恒斐生物科技有限公司

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上海恒斐生物科技有限公司
刘鑫
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845206284@qq.com
845206284
产品属性
英文名BcuI (SpeI) (10 U/µL)
数量现货
供应商恒斐生物
CAS号相关应用： 限制性内切酶和克隆酶
规格2000 units
产品说明

BcuI (SpeI) (10 U/µL)描述

5'A ↓C T A G T 3'
3'T G A T C ↑A 5'

Thermo Scientific BcuI (SpeI) restriction enzyme recognizes A^CTAGT sites and cuts best at 37°C in Tango buffer (isoschizomers: AhlI, SpeI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

BcuI (SpeI) (10 U/µL)规格

Enzyme:BcuI (SpeI)
Product Size:2,000 units
Compatible Buffer:10x Buffer Tango
Methylation Sensitivity:Not dam methylation-sensitive, Not CpG methylation-sensitive, Not dcm methylation-sensitive
Optimal Reaction Temperature:37° C
Sensitive to Heat Inactivation:Yes
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