Kit Summary:
• Detection method- Absorbance (450 nm)
• Sample type- Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, fermentation media
• Species reactivity- Mammalian
• Application- The useful concentration range in samples is 0.01 mM – 10 mM D-lactate.

Features & Benefits:
• Simple procedure; takes ~ 40 minutes
• Fast and convenient
• Kit contains all necessary reagents for accurate measurement of Lactate

Kit components:
• D-Lactate Assay Buffer
• D-Lactate Enzyme Mix
• D-Lactate Substrate Mix
• D-Lactate Standard (100 mM)

Description:
D-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. Abnormally high concentrations of D-lactate are considered indicative of sepsis, ischemia or trauma. Due to slow metabolism and excretion, high D-lactate can cause acidosis and encephalopathy. BioVision’s D-Lactate Assay Kit provides a fast, easy way to accurately measure D-lactate in a variety of biological samples. In the D-Lactate Assay Kit, D-lactate is specifically oxidized by D-lactate dehydrogenase and generates proportional color (λmax = 450 nm). The kit detects D-Lactate in samples such as serum, plasma, cells, culture and fermentation media. The useful concentration range in samples is 0.01 mM – 10 mM D-lactate.

Storage Conditions:
-20°C

Shipping Conditions:
gel pack

USAGE: For Research Use Only! Not For Use in Humans.