产品名称:LDH细胞毒性检测试剂盒
英文名称:LDH Cytotoxicity Assay
货号:8078
品牌:ScienCell
产地:美国
规格:500 Tests
Description
Lactate dehydrogenase (LDH), which is a soluble cytosolic enzyme present in most eukaryotic cells, releases
into culture medium upon cell death due to damage of plasma membrane. The increase of the LDH activity in
culture supernatant is proportional to the number of lysed cells. ScienCell LDH Cytotoxicity Assay kit provides
a colorimetric method to measure LDH activity using a reaction cocktails containing lactate, NAD+, diaphrose
and
INT. LDH catalyses the reduction of NAD+ to NADH in the presence of L-lactate, while the formation of NADH can be measured in a coupled reaction in which the tetrazolium salt INT
is reduced to a red formazan product. The amount of the highly colored and soluble formazan can be
measured at 490 nm spectrophotometrically.
Product Use
This assay kit is used to evaluate LDH activity in vitro. It is for research use only. Not for use in animals,
humans, or diagnostic procedures.