本公司提供科研Nanog胚胎干细胞关键蛋白抗体，抗体质量可靠，订购Nanog胚胎干细胞关键蛋白抗体请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：胚胎干细胞关键蛋白抗体
英文名称：Anti-Nanog
货号：bs-0829R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 34kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, rat, mo
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：Nanog is a newly identified homeodomain-bearing transcriptional factor. Nanog expression is specific to early embryos and pluripotential stem cells including mouse and human embryonic stem (ES) and embryonic germ (EG) cells. It is a key molecule involved in the signaling pathway for maintaining the capacity for self-renewal and pluripotency, bypassing regulation by the STAT3 pathway. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Nanog-deficient ES cells lose pluripotency and differentiate into extraembryonic endoderm lineage. Thus it is one of the molecular markers suitable for recognizing the undifferentiated state of stem cells in the mouse and human. NANOG is a new marker for testicular carcinoma in situ and germ cell tumors.NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).Subunit : Interacts with SMAD1 and SALL4 (By similarity).Subcellular Location : Nucleus.Tissue Specificity : Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.Similarity : Belongs to the Nanog homeobox family. Contains 1 homeobox DNA-binding domain. 研究表明；早期胚胎在没有Nanog存在的情况下是不能存活的，但是这一蛋白的表达水平至今还有待于进一步研究。学者认为：Nanog依然是一种对于生殖细胞（germ lls）形成的必要因子，Nanog也是调控着细胞融合后的多重功能的关键因子。 胚胎干细胞是当今生命科学和生物技术研究的热点，这主要是由于其具有“发育全能性”的功能。ES能分化成人体200多种细胞类型，形成机体的任何细胞、组织和器官。通过掌握其分化发育的规律，在人工条件下定向分化为所需的细胞、组织乃至器官，科学家们希望可以用来治疗目前还难以或无法治愈的帕金森氏病、早老性痴呆、白血病、糖尿病等顽症，并且解决十分紧缺的组织和器官移植的来源问题，并且通过进一步与克隆技术相结合，运用体细胞核转移技术来得到ES，还能解决细胞治疗以及组织和器官移植的免疫排异难题。产品图片Tissue/cell: Mouse tooth germ tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Nanog Polyclonal Antibody, Unconjugated(bs-0829R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingProtein: line1, human colon carcinoma lysates, 30ug; line2, rat brain lysates, 30ug; Primary: Anti-Nanog (bs-0829R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 34kDObserved band size : 49kDWe are unsure as to the identity of these extra bands