黑素瘤细胞黏附分子(MCAM)重组蛋白Recombinant Melanoma Cell Adhesion Molecule (MCAM)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 90% |
| UOM | 50ug |
| Predicted Molecular Mass | 29.4kDa |
| Concentration | n/a |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Gln323~Lys560 with two N-terminal Tags, His-tag and T7-tag |
黑素瘤细胞黏附分子(MCAM)重组蛋白| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The sequence of the target protein is listed below.
QSLDLETT VMLSSDPLEL LVNYVSDVQV DPTAPEVQEG DSLTLTCKAE SNQDLEFEWL RDKTGQLLGK GPILQLNNVK REAGGRYLCV ASVPSVPGLN RTRRVSVGIF GSPWMAAKER KVWAQENAML NLSCEASGHP QPTISWNING SATEWNPDPQ TVVSTLNVLV TPELLETGAE CTASNSLGSN TTVIILKLVT LTTLTPDSSQ TTGLSTPTVS PHSRANSTST EKKLPQQESK
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
黑素瘤细胞黏附分子(MCAM)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
黑素瘤细胞黏附分子(MCAM)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
