人血管生成素1(ANGPT1)检测试剂盒

¥2988
人血管生成素1(ANGPT1)检测试剂盒
国产
2021-08-30 09:19

上海恒斐生物科技有限公司

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上海恒斐生物科技有限公司
刘鑫
17317793691 021-61843644
845206284@qq.com
845206284
产品属性
数量大量
英文名ELISA Kit for Angiopoietin 1 (ANGPT1)
CAS号产品通过ISO 认证 有大量文献发表
保质期6个月
供应商上海恒斐生物科技有限公司
保存条件2-8度
规格96T
产品说明
人血管生成素1(ANGPT1)检测试剂盒 产品通过ISO 认证 有大量文献发表
  • 实验方法双抗夹心
  • 反应时长3h
  • 检测范围0.24-15ng/mL
  • 灵敏度最小可检测剂量小于等于0.11ng/mL

实验流程

1. 实验前标准品、试剂及样本的准备;
2. 加样(标准品及样本)100µL,37°C孵育1小时;
3. 吸弃,加检测溶液A100µL,37°C孵育1小时;
4. 洗板3次;
5. 加检测溶液B100µL,37°C孵育30分钟;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分钟;
8. 加终止液50µL,立即450nm读数。

实验原理

将血管生成素1(ANGPT1)抗体包被于96孔微孔板中,制成固相载体,向微孔中分别加入标准品或标本,其中的血管生成素1(ANGPT1)与连接于固相载体上的抗体结合,然后加入生物素化的血管生成素1(ANGPT1)抗体,将未结合的生物素化抗体洗净后,加入HRP标记的亲和素,再次彻底洗涤后加入TMB底物显色。TMB在过氧化物酶的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管生成素1(ANGPT1)呈正相关。用酶标仪在450nm波长下测定吸光度(O.D.值),计算样品浓度。

参考文献

杂志参考文献
TheriogenologyEvaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article]
J Atheroscler ThrombHigh Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction [PubMed: 25735431]
Archives of Biological SciencesDiabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu]
Am J Transl ResAngiopoietin-1 facilitates recovery of hematopoiesis in radiated mice [Pubmed:27347310]
Clinical researchIncreased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19]
Turkish Journal of Medicl SciencesA new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer [pubmed:28425270]
Sci Rep.Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491]
PeerJThe ratio of serum Angiopoietin-1 to Angiopoietin-2 in patients with cervical cancer is a valuable diagnostic and prognostic biomarker [pubmed:28584715]
Molecular&cellular proteomicsPrediction of Response to Sorafenib in Hepatocellular Carcinoma: A Marker Panel by Multiple Reaction Monitoring-Mass Spectrometry [pubmed:28550167]
Tumor BiologyCervical cancer cell–derived angiopoietins promote tumor progression [pubmed:28720059]
Free Patents onlineRHEUMATOID ARTHRITIS RELATED BIOMARKER [20170350884]
AnesthesiologyVasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in … [Pubmed:28968277]
British Journal of AnaesthesiaVasculotide, an angiopoietin-1 mimetic, reduces pulmonary vascular leakage and preserves microcirculatory perfusion during cardiopulmonary bypass in rats [10.1016:j.bja.2018.05.049]
The Journal of Biological ChemistryToll-like receptor 2 deficiency hyperactivates the FoxO1 transcription factor and induces aging-associated cardiac dysfunction in mice [Pubmed:29929978]
Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse [Doi: 10.4143/crt.2018.214]
BIOLOGY OF REPRODUCTIONProstaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors [Pubmed: 30929911]