Application
Determination of ATP in ATP-generating or ATP-consuming reactions. Determination of the activity of enzymes such as creatine kinase and ATPases. Under optimal conditions, 1 photon is released per molecule ATP in the bioluminescence assay.
One mg is sufficient for approximately 5,000 ATP determinations in the concentration range of 0.1 nM to 1 μM.
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
Storage conditions (working solution): -15 to -25 °C
Store the reconstituted solution in aliquots at -15 to -25 °C.
Avoid repeated freezing and thawing.
Information Note: Do not store in plastic vessels.
Quality
Contaminants: <1 mU ATPase/mg lyophilizate, and <10 mU nucleoside diphosphatase kinase/mg lyophilizate. The lyophilizate contains neither luciferin nor arsenate.
Reconstitution
Cool the original glass vial (1 mg or 5 mg protein, respectively) to 0 °C in an ice-bath and dissolve the containing luciferase by adding 1 ml or 5 ml, respectively, 0.5 M ice-cold Tris-acetate buffer, pH 7.5, and let it stand for 10 minutes. (The dissolved enzyme is sensitive to shaking and to storage in plastic vessels). This concentrated solution is stable for several
months when stored frozen in portions of approximately 50 μl. Diluted solutions are unstable and should be prepared directly before use and should be kept in an ice-bath.
One microgram luciferase corresponds to approximately 107 arbitrary luciferase units.
