Mouse Surfactant Protein D, SP- D ELISA kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,surfactant protein DCOLEC7, PSPD,SFTP4, SP-D, collectin 7, collectin-7, lung surfactant protein D, pulmonary surfactant apoprotein, pulmonary surfactant-associated protein D, surfactant pulmonary-associated protein D, surfactant-associated protein pulmonary 4, COLEC7,CP4

Search name

Mouse Pulmonary surfactant-associated protein D ELISA KIT ,Mouse PSP-D ELISA KIT ,Mouse SP-D ELISA KIT , Mouse PSPD

ELISA KIT, Mouse Collectin-7 ELISA KIT ,Mouse Lung surfactant protein D ELISA KIT ,Mouse surfactant protein DCOLEC7

ELISA KIT , Mouse SFTP4 ELISA KIT ,Mouse SP-D ELISA KIT ,Mouse collectin 7 ELISA KIT ,Mouse collectin-7 ELISA

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surfactant-associated protein pulmonary 4 ELISA KIT,Mouse COLEC7 ELISA KIT,Mouse CP4 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of mouse pulmonary surfactant-associated protein d,PSP-D concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to PSP-D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PSP-D and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PSP-D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PSP-D in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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