品牌:CST，产品名称：CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881
英文名称：CD3 (UCHT1) Mouse mAb (APC Conjugate)
Product name：CD3 (UCHT1) Mouse mAb (APC Conjugate) #19881
Applications：F ,
Purification： This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background： When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5).The UCHT1 antibody is widely used as a marker for phenotyping human T cells (6-8).

Entrez-Gene Id

Swiss-Prot Acc.

Specificity / Sensitivity:

CD3 (UCHT1) Mouse mAb (APC Conjugate) recognizes endogenous levels of total CD3ε, CD3γ, and CD3δ proteins. This antibody detects epitopes within both the extracellular and intracellular domains of CD3ε.

Species Reactivity:

Human

Storage:

Supplied in 10 mM NaH₂PO₄, 150 mM NaCl, 0.09% NaN₃, 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Product Description:

This Cell Signaling Technology antibody is conjugated to allophycocyanin (APC) and tested in-house for direct flow cytometric analysis in human cells.