活细胞染色 CytoTell 绿色

¥1200
AAT Bioquest
进口
2021-09-01 18:46

上海齐源生物科技有限公司

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上海齐源生物科技有限公司
杨阳
15021410950
3123991360@qq.com
产品属性
数量大量
英文名CytoTell™ UltraGreen
CAS号/
保质期详见说明
供应商齐源生物
保存条件详见说明
规格500 Tests
产品说明
齐源生物专业代理美国AAT Bioquest产品,并有大量储存现货欢迎致电:021-59588506 
关键词:活细胞染色 CytoTell 绿色详细如下
Flow cytometry combined with fluorescence staining is a powerful tool to analyze heterogeneous cell populations. Among all the existing fluorescent dyes CFSE is the preferred cell proliferation indicator that is widely used for live cell analysis. However, there are a few severe problems associated with the use of CFSE for monitoring cell proliferation. 1). CFSE is highly toxic to cells. CFSE indiscriminately reacts with all amino groups, thus changes many critical intracellular protein functions (such as cell membrane GPCRs); 2). CFSE has slow response and is inconvenient to use. The CFSE fluorescence intensity of the 2nd generation cells is decreased more than 10 fold from the 1st generation. You would have to wait for another generation to start the cell proliferation analysis. 3). Medium removal is required. You would have to remove medium for cell analysis with a flow cytometer since CFSE reacts with medium components. CytoTell™ Green has been developed to eliminate these CFSE limitations. Based on our customers' feedbacks on our CytoTell™ Green, CytoTell™ UltraGreen is our newest improvement. It has distinct advantages. 1). CytoTell™ UltraGreen is well retained in cells; 2). CytoTell™ UltraGreen exhibits much faster response and is more convenient to use than CFSE. The fluorescence intensity gap between 1st and 2nd generation is significantly minimized. As cells divide, CytoTell™ UltraGreen is distributed equally between daughter cells that can be measured as successive halving of the fluorescence intensity of the dye; 3). CytoTell™ UltraGreen is more sensitive than CFSE. Up to 9 generations may be visualized; 4). CytoTell™ UltraGreen is much more stable than CFSE. CytoTell™ UltraGreen stock solution can be stored at room temperature for a few days. CytoTell™ UltraGreen can also be used for long term tracking of labeled cells. Analysis using two-parameter plots may provide better resolution of each generation, especially between undivided cells and the first generation. CytoTell™ UltraGreen has a peak excitation of 519 nm and can be excited by the blue (488 nm) laser line, making it compatible with FITC filter set.
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