中断位点蛋白STIL抗体图片价格,详情介绍-960化工网

中断位点蛋白STIL抗体图片

价格：¥1000 - 3500

品牌：上海博湖

产地：进口、国产

最新更新日期：2021-09-01 23:32

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上海博湖生物科技有限公司

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公司：上海博湖生物科技有限公司

联系人：韩丽君

联系电话： 18117197628 021-57763112

联系邮箱：3004987436@qq.com

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产品属性

抗体名	中断位点蛋白STIL抗体图片

抗体英文名

Anti-STIL

靶点

详见说明书

浓度

1mg/1ml

应用范围	WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 IF=1:50-200

宿主

详见说明书

供应商

上海博湖

数量

大量

级别

详见说明书

目录编号

详见说明书

抗原来源

Rabbit

保质期

详见说明书

适应物种

详见说明书

标记物

详见说明书

克隆性

多克隆

保存条件	Store at -20 °C

形态

详见说明书

亚型

IgG

免疫原	KLH conjugated synthetic peptide derived from human STIL

规格	0.2ml/200μg

产品说明

英文名称 Anti-STIL
中文名称中断位点蛋白STIL抗体图片
别名 MCPH7; SCL interrupting locus protein; SCL-interrupting locus protein; SCL/TAL1 interrupting locus; SIL; STIL; STIL_HUMAN; TAL 1 interrupting locus protein; TAL-1-interrupting locus protein.

中断位点蛋白STIL抗体图片浓度 1mg/1ml
规格 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Cow, Rabbit
产品类型一抗
研究领域肿瘤细胞生物免疫学神经生物学信号转导干细胞细胞周期蛋白细胞分化
蛋白分子量 predicted molecular weight: 143kDa
性状 Lyophilized or Liquid
免疫原 KLH conjugated synthetic peptide derived from human STIL
亚型 IgG
纯化方法 affinity purified by Protein A
储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

中断位点蛋白STIL抗体图片产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-100 IF=1:50-200
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 TAL1 disruption at 1p32, a common rearrangement in the T-cell acute lymphoblastic leukemia, usually results in the formation of a SCL interrupting locus (SIL)-TAL1 fusion product. SIL is an immediate early gene whose expression is associated with cell proliferation. The Sil protein exhibits ubiquitous expression in hematopoietic cell lines and tissues. However, Sil protein levels remain tightly regulated during the cell cycle, achieving peak levels in mitosis and diminishing on transition to G1 phase. Overexpression of Sil in primary adenocarcinomas predicts metastatic spread, especially in lung tumors with increased mitotic activity.
Function : Immediate-early gene. Plays an important role in embryonic development as well as in cellular growth and proliferation; its long-term silencing affects cell survival and cell cycle distribution as well as decreases CDK1 activity correlated with reduced phosphorylation of CDK1. Plays a role as a positive regulator of the sonic hedgehog pathway, acting downstream of PTCH1.
Subunit : Interacts with PIN1 via its WW domain. This interaction is dependent on STIL mitotic phosphorylation (By similarity).
Subcellular Location : Cytoplasm, cytosol.
Tissue Specificity : Expressed in all hematopoietic tissues and cell lines. Highly expressed in a variety of tumors characterized by increased mitotic activity with highest expression in lung cancer.
Post-translational modifications : Phosphorylated following the activation of the mitotic checkpoint.
DISEASE : Note=A chromosomal aberration involving STIL may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). A deletion at 1p32 between STIL and TAL1 genes leads to STIL/TAL1 fusion mRNA with STIL exon 1 slicing to TAL1 exon 3. As both STIL exon 1 and TAL1 exon 3 are 5'-untranslated exons, STIL/TAL1 fusion mRNA predicts a full length TAL1 protein under the control of the STIL promoter, leading to inappropriate TAL1 expression. In childhood T-cell malignancies (T-ALL), a type of defect such as STIL/TAL1 fusion is associated with a good prognosis. In cultured lymphocytes from healthy adults, STIL/TAL1 fusion mRNA may be detected after 7 days of culture.
Defects in STIL are the cause of microcephaly primary type 7 (MCPH7) [MIM:612703]. Microcephaly is defined as a head circumference more than 3 standard deviations below the age-related mean. Brain weight is markedly reduced and the cerebral cortex is disproportionately small. Despite this marked reduction in size, the gyral pattern is relatively well preserved, with no major abnormality in cortical architecture. Primary microcephaly is further defined by the absence of other syndromic features or significant neurological deficits.
Database links : UniProtKB/Swiss-Prot: Q15468.2

中断位点蛋白STIL抗体图片抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体抗体的交叉反应：人，小鼠，大鼠，鸡，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。
中断位点蛋白STIL抗体图片实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。

ER- Alpha/ER alpha peptide 雌激素受体α抗原Multi-class antibodies规格： 0.5mg
化相关死亡促进因子抗体 Anti-phospho-BAD 0.1ml
Rhesus antibody Rh phospho-EGFR (Thr678) 化表皮生长因子受体抗体规格 0.1ml
MEM 10×1L Gibco
ZNF449 英文名称：指蛋白449抗体 0.2ml
Dnmt3a 英文名称： DNA甲基转移酶-3α抗体 0.1ml
化相关死亡促进因子抗体 Anti-phospho-BAD 0.1ml
ATEXPA10 peptide 植物扩张蛋白ATEXPA10(拟南芥)抗原Multi-class antibodies规格： 0.5mg
抗化Bcl-xL(pSer62)蛋白抗体 Anti-phospho-Bcl-xL 0.1ml
Rhesus antibody Rh phospho-EGFR (Se66) 化表皮生长因子受体抗体规格 0.1ml
DMEM高糖 10×1L Gibco
ZNF7 英文名称：指蛋白7抗体 0.2ml
DYRK3 英文名称：双特异性酪酸化调节激酶3抗体 0.2ml
抗化Bcl-xL(pSer62)蛋白抗体 Anti-phospho-Bcl-xL 0.1ml
ZnT-1 英文名称：转运蛋白1抗体 0.2ml
DHRS2 英文名称：脱酶/还原酶家族成员2抗体 0.1ml
化APP(Tyr757)淀粉样肽前体蛋白抗体 Anti-phospho-APP 0.1ml
Anti-AFP/Biotin 生物素化鼠抗人甲胎蛋白单克隆抗体(检测)IgGMulti-class antibodies规格： 0.2ml
Rhesus antibody Rh Phospho-eNOS(Se77) 化合成酶3抗体(内皮型) 规格 0.1ml
E2F1 转录因子E2F-1Multi-class antibodies规格： 0.5mg
In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.
Function : In the process of endocytosis, essential rate-limiting regulator of a fast recycling pathway back to the plasma membrane. During cytokinesis, required for the postfurrowing terminal steps, namely for intercellular bridge stability and abscission, possibly by controlling phosphatidylinositol 4,5-bis phosphate (PIP2) and SEPT2 localization at the intercellular bridge.
Subunit : Interacts with DENND1A and DENND1B in a nucleotide-dependent manner. The low binding to DENND1C is nucleotide-independent. All 3 DENND1 act as GEFs for RAB35 and thus activate the protein.
Subcellular Location : Cell membrane; Lipid-anchor; Cytoplasmic side (Potential). Membrane, clathrin-coated pit. Cytoplasmic vesicle, clathrin-coated vesicle. Endosome. Melanosome.
Post-translational modifications : AMPylation at Tyr-77 by L.pneumophila DrrA occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB35 constitutively active.
Phosphocholinated by L.pneumophila AnkX. Both GDP-bound and GTP-bound forms can be phosphocholinated. Phosphocholination inhibits the GEF activity of DENND1A.
Similarity : Belongs to the small GTPase superfamily. Rab .
Anti-CD4/Biotin 生物素化CD4抗体IgGMulti-class antibodies规格： 0.2ml
Pentapeptide-3/Leuphasyl 五胜肽Multi-class antibodies规格： 1g
细胞外基质金属蛋白酶诱导因子抗体 Anti-CD147/EMMPRIN 0.1ml
SYK 英文名称：非受体型酪酸蛋白激酶抗体 0.1ml
EphA3 英文名称：酪酸蛋白激酶受体A3抗体 0.1ml
Rhesus antibody Rh phospho-HDAC1(Ser423) 化组蛋白去乙酰化酶1抗体规格 0.1ml
Pentapeptide-3/Leuphasyl 五胜肽Multi-class antibodies规格： 1g
中断位点蛋白STIL抗体图片Shiga-like toxin IIe variant subunit A[Escherichia coli 0139] 大肠杆菌志贺样毒素菌体蛋白(O139菌型)Multi-class antibodies规格： 0.5mg
Anti-Bid BH3结构域凋亡诱导蛋白抗体Multi-class antibodies规格： 0.2ml
Rhesus antibody Rh PGGT1β香叶烯基转移酶1β抗体规格 0.2ml
Fas Ligand 浓缩液 0.1ml 进口分装
UFD1L 英文名称：泛素融合降解样蛋白1抗体 0.2ml
CCL23/MIP3 英文名称：巨噬细胞炎性蛋白抗体 0.1ml
Anti-Bid BH3结构域凋亡诱导蛋白抗体Multi-class antibodies规格： 0.2ml