SepMate™ - Specialized PBMC Isolation Tube
熟悉PBMC分离的人员都知道密度梯度分层的难点在于:离心过程中加样过快、离心管不平衡或打开刹车引起的分层不清晰,都会影响您的实验效果。
如果您的样品是人全血,脐血或者骨髓的,建议推荐SepMate这个产品
http://www.stemcell.com/en/Products/All-Products/SepMate.aspx
这个产品的最大优势是让原本近1个小时的Ficoll提PBMC的操作减少到15分钟,如果和RosetteSep配合使用,只需25分钟就可以完成对目标细胞的预富集。
SepMate™ is a new tube that enables consistent and hassle-free PBMC isolations in just 15 minutes. The unique SepMate™ insert lets you quickly layer blood over the density gradient medium (e.g. Ficoll-Paque™, Lymphoprep™), and prevents the layers from mixing. After centrifugation with the brake on, PBMCs are simply poured into a fresh tube.
PIS - http://www.stemcell.com/~/media/Technical%20Resources/6/D/4/9/D/29251PIS_1_1_0.ashx
SepMate使用视频:http://www.stemcell.com/en/Technical-Resources/dedb8/SepMate-Hassle-Free-PBMCs-in-Just-15-Minutes.aspx