GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates
Specificity:
Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP
Purification:
Protein A affinity chromatography from mouse ascites fluid
Applications:
Dot, ELISA, IP, IS, WB
Sensitivity:
WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins
Working Condition:
WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:
Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
Fig. 1:1000 (1μg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1μg (2) and 10μg (3) of cell lysates used
